Cargando…
An optimized protocol to analyze membrane protein degradation in yeast using quantitative western blot and flow cytometry
Membrane proteins (MPs) are essential in many cellular functions. To maintain proteostasis, MPs are downregulated via ubiquitination and degradation. Here, we describe an optimized protocol to analyze MP degradation using quantitative western blot and flow cytometry-based approaches. We use the degr...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987394/ https://www.ncbi.nlm.nih.gov/pubmed/35403002 http://dx.doi.org/10.1016/j.xpro.2022.101274 |