Label-Free Characterization of Amyloids and Alpha-Synuclein Polymorphs by Exploiting Their Intrinsic Fluorescence Property

[Image: see text] Conventional in vitro aggregation assays often involve tagging with extrinsic fluorophores, which can interfere with aggregation. We propose the use of intrinsic amyloid fluorescence lifetime probed using two-photon excitation and represented by model-free phasor plots as a label-f...

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Detalles Bibliográficos
Autores principales: Chung, Chyi Wei, Stephens, Amberley D., Ward, Edward, Feng, Yuqing, Davis, Molly Jo, Kaminski, Clemens F., Kaminski Schierle, Gabriele S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988127/
https://www.ncbi.nlm.nih.gov/pubmed/35333515
http://dx.doi.org/10.1021/acs.analchem.1c05651

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988127/
https://www.ncbi.nlm.nih.gov/pubmed/35333515
http://dx.doi.org/10.1021/acs.analchem.1c05651

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