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Molecular characterization of ethyl carbamate toxicity in Caenorhabditis elegans
Ethyl carbamate is a common contaminant prevalent in fermented food with probable carcinogenic effects in animals. To date, other toxicological properties of ethyl carbamate are not well characterized. Using the genetic model Caenorhabditis elegans, we found that chronic exposure to ethyl carbamate...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990054/ https://www.ncbi.nlm.nih.gov/pubmed/35399212 http://dx.doi.org/10.1016/j.toxrep.2022.03.029 |
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author | Comfort, Jordan J. Chomyshen, Samantha C. Waddell, Brandon M. Tabarraei, Hadi Wu, Cheng-Wei |
author_facet | Comfort, Jordan J. Chomyshen, Samantha C. Waddell, Brandon M. Tabarraei, Hadi Wu, Cheng-Wei |
author_sort | Comfort, Jordan J. |
collection | PubMed |
description | Ethyl carbamate is a common contaminant prevalent in fermented food with probable carcinogenic effects in animals. To date, other toxicological properties of ethyl carbamate are not well characterized. Using the genetic model Caenorhabditis elegans, we found that chronic exposure to ethyl carbamate during larval development impedes growth while exposure during adulthood inhibits reproduction, shortens lifespan, and promotes degeneration to dopaminergic neurons. Through whole-transcriptome RNA-sequencing, we found that ethyl carbamate invokes a widespread transcriptomic response inducing the differential expression of > 4,000 genes by at least 2-fold. Functional analysis of RNA-sequencing data revealed that up-regulated genes enrich to various neuron regulatory processes and xenobiotic defense. Gene expression analysis confirms that various genes encoding antioxidant enzymes and those functioning within phase I and II detoxification responses along with ABC transporters are highly up-regulated after ethyl carbamate exposure, suggesting the onset of oxidative stress. Overall, these findings report new toxicological properties of chronic ethyl carbamate exposure and provide new insights on its effects on transcriptome regulation in the C. elegans model. |
format | Online Article Text |
id | pubmed-8990054 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-89900542022-04-09 Molecular characterization of ethyl carbamate toxicity in Caenorhabditis elegans Comfort, Jordan J. Chomyshen, Samantha C. Waddell, Brandon M. Tabarraei, Hadi Wu, Cheng-Wei Toxicol Rep Regular Article Ethyl carbamate is a common contaminant prevalent in fermented food with probable carcinogenic effects in animals. To date, other toxicological properties of ethyl carbamate are not well characterized. Using the genetic model Caenorhabditis elegans, we found that chronic exposure to ethyl carbamate during larval development impedes growth while exposure during adulthood inhibits reproduction, shortens lifespan, and promotes degeneration to dopaminergic neurons. Through whole-transcriptome RNA-sequencing, we found that ethyl carbamate invokes a widespread transcriptomic response inducing the differential expression of > 4,000 genes by at least 2-fold. Functional analysis of RNA-sequencing data revealed that up-regulated genes enrich to various neuron regulatory processes and xenobiotic defense. Gene expression analysis confirms that various genes encoding antioxidant enzymes and those functioning within phase I and II detoxification responses along with ABC transporters are highly up-regulated after ethyl carbamate exposure, suggesting the onset of oxidative stress. Overall, these findings report new toxicological properties of chronic ethyl carbamate exposure and provide new insights on its effects on transcriptome regulation in the C. elegans model. Elsevier 2022-04-04 /pmc/articles/PMC8990054/ /pubmed/35399212 http://dx.doi.org/10.1016/j.toxrep.2022.03.029 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Regular Article Comfort, Jordan J. Chomyshen, Samantha C. Waddell, Brandon M. Tabarraei, Hadi Wu, Cheng-Wei Molecular characterization of ethyl carbamate toxicity in Caenorhabditis elegans |
title | Molecular characterization of ethyl carbamate toxicity in Caenorhabditis elegans |
title_full | Molecular characterization of ethyl carbamate toxicity in Caenorhabditis elegans |
title_fullStr | Molecular characterization of ethyl carbamate toxicity in Caenorhabditis elegans |
title_full_unstemmed | Molecular characterization of ethyl carbamate toxicity in Caenorhabditis elegans |
title_short | Molecular characterization of ethyl carbamate toxicity in Caenorhabditis elegans |
title_sort | molecular characterization of ethyl carbamate toxicity in caenorhabditis elegans |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990054/ https://www.ncbi.nlm.nih.gov/pubmed/35399212 http://dx.doi.org/10.1016/j.toxrep.2022.03.029 |
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