Spacer2PAM: A computational framework to guide experimental determination of functional CRISPR-Cas system PAM sequences

RNA-guided nucleases from CRISPR-Cas systems expand opportunities for precise, targeted genome modification. Endogenous CRISPR-Cas systems in many prokaryotes are attractive to circumvent expression, functionality, and unintended activity hurdles posed by heterologous CRISPR-Cas effectors. However,...

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Detalles Bibliográficos
Autores principales: Rybnicky, Grant A, Fackler, Nicholas A, Karim, Ashty S, Köpke, Michael, Jewett, Michael C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990532/
https://www.ncbi.nlm.nih.gov/pubmed/35258601
http://dx.doi.org/10.1093/nar/gkac142

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990532/
https://www.ncbi.nlm.nih.gov/pubmed/35258601
http://dx.doi.org/10.1093/nar/gkac142

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