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Imaging Unique DNA Sequences in Individual Cells Using a CRISPR-Cas9-Based, Split Luciferase Biosensor

An extensive arsenal of biosensing tools has been developed based on the clustered regularly interspaced short palindromic repeat (CRISPR) platform, including those that detect specific DNA sequences both in vitro and in live cells. To date, DNA imaging approaches have traditionally used full fluore...

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Detalles Bibliográficos
Autores principales: Heath, Nicholas G., O’Geen, Henriette, Halmai, Nicole B., Corn, Jacob E., Segal, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990833/
https://www.ncbi.nlm.nih.gov/pubmed/35403097
http://dx.doi.org/10.3389/fgeed.2022.867390