A detailed protocol for expression, purification, and activity determination of recombinant SaCas9

Recombinant SaCas9 is useful for a broad range of applications in the context of genome editing, especially when the specific protospacer adjacent motifs of other Cas9 derivatives are missing. Here, we describe a detailed protocol for the expression and purification of recombinant SaCas9. We detail...

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Detalles Bibliográficos
Autores principales: Flottmann, Franziska, Pohl, Greta Marie, Gummert, Jan, Milting, Hendrik, Brodehl, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991255/
https://www.ncbi.nlm.nih.gov/pubmed/35403007
http://dx.doi.org/10.1016/j.xpro.2022.101276

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991255/
https://www.ncbi.nlm.nih.gov/pubmed/35403007
http://dx.doi.org/10.1016/j.xpro.2022.101276

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