Phase-In to Phase-Out—Targeted, Inclusive Strategies Are Needed to Enable Full Replacement of Animal Use in the European Union

SIMPLE SUMMARY: In the European Union (and elsewhere), the overall use of animals in laboratories has failed to undergo any significant decline, despite six decades of purported adherence to the “3Rs” principles of replacement, reduction, and refinement. In the EU, the 1986 adoption of a legal requirement to use scientific methods not entailing the use of live animals, rising public opinion against the use of animals and the almost exponential rise in development and application of non-animal new approach methodologies (NAMs) signals a readiness to end animal testing. Indeed, the European Parliament recently carried an almost unanimous vote to adopt an action plan to phase out the use of animals in research and testing. This article explores what is needed to make this action plan a success, considering all stakeholders and their needs. ABSTRACT: In September 2021, the European Parliament voted overwhelmingly in favour of a resolution to phase out animal use for research, testing, and education, through the adoption of an action plan. Here we explore the opportunity that the action plan could offer in developing a more holistic outlook for fundamental and biomedical research, which accounts for around 70% of all animal use for scientific purposes in the EU. We specifically focus on biomedical research to consider how mapping scientific advances to patient needs, taking into account the ambitious health policies of the EU, would facilitate the development of non-animal strategies to deliver safe and effective medicines, for example. We consider what is needed to help accelerate the move away from animal use, taking account of all stakeholders and setting ambitious but realistic targets for the total replacement of animals. Importantly, we envisage this as a ‘phase-in’ approach, encouraging the use of human-relevant NAMs, enabling their development and application across research (with applications for toxicology testing). We make recommendations for three pillars of activity, inspired by similar efforts for making the shift to renewable energy and reducing carbon emissions, and point out where investment—both financial and personnel—may be needed.