Sequencing of individual barcoded cDNAs using Pacific Biosciences and Oxford Nanopore Technologies reveals platform-specific error patterns

Long-read transcriptomics require understanding error sources inherent to technologies. Current approaches cannot compare methods for an individual RNA molecule. Here, we present a novel platform-comparison method that combines barcoding strategies and long-read sequencing to sequence cDNA copies re...

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Detalles Bibliográficos
Autores principales: Mikheenko, Alla, Prjibelski, Andrey D., Joglekar, Anoushka, Tilgner, Hagen U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2022
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997348/
https://www.ncbi.nlm.nih.gov/pubmed/35301264
http://dx.doi.org/10.1101/gr.276405.121

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997348/
https://www.ncbi.nlm.nih.gov/pubmed/35301264
http://dx.doi.org/10.1101/gr.276405.121

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