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Sequencing of individual barcoded cDNAs using Pacific Biosciences and Oxford Nanopore Technologies reveals platform-specific error patterns

Long-read transcriptomics require understanding error sources inherent to technologies. Current approaches cannot compare methods for an individual RNA molecule. Here, we present a novel platform-comparison method that combines barcoding strategies and long-read sequencing to sequence cDNA copies re...

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Detalles Bibliográficos
Autores principales:	Mikheenko, Alla, Prjibelski, Andrey D., Joglekar, Anoushka, Tilgner, Hagen U.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2022
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997348/ https://www.ncbi.nlm.nih.gov/pubmed/35301264 http://dx.doi.org/10.1101/gr.276405.121

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