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Effect of Phenolic Extract from Red Beans (Phaseolus vulgaris L.) on T-2 Toxin-Induced Cytotoxicity in HepG2 Cells

Red beans contain human bioactive compounds such as polyphenols. Several in vitro studies have proposed the natural compounds as an innovative strategy to modify the toxic effects produced by mycotoxins. Hence, in this work, a complete investigation of the polyphenolic fraction of red beans was perf...

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Autores principales: Martínez-Alonso, Carmen, Taroncher, Mercedes, Castaldo, Luigi, Izzo, Luana, Rodríguez-Carrasco, Yelko, Ritieni, Alberto, Ruiz, María-José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997370/
https://www.ncbi.nlm.nih.gov/pubmed/35407120
http://dx.doi.org/10.3390/foods11071033
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author Martínez-Alonso, Carmen
Taroncher, Mercedes
Castaldo, Luigi
Izzo, Luana
Rodríguez-Carrasco, Yelko
Ritieni, Alberto
Ruiz, María-José
author_facet Martínez-Alonso, Carmen
Taroncher, Mercedes
Castaldo, Luigi
Izzo, Luana
Rodríguez-Carrasco, Yelko
Ritieni, Alberto
Ruiz, María-José
author_sort Martínez-Alonso, Carmen
collection PubMed
description Red beans contain human bioactive compounds such as polyphenols. Several in vitro studies have proposed the natural compounds as an innovative strategy to modify the toxic effects produced by mycotoxins. Hence, in this work, a complete investigation of the polyphenolic fraction of red beans was performed using a Q-Orbitrap high-resolution mass spectrometry analysis. Notably, epicatechin and delphinidin were the most detected polyphenols found in red bean extracts (3.297 and 3.108 mg/Kg, respectively). Moreover, the red bean extract was evaluated against the T-2 toxin (T-2) induced cytotoxicity in hepatocarcinoma cells (HepG2) by direct treatment, simultaneous treatment, and pre-treatment assays. These data showed that T-2 affected the cell viability in a dose-dependent manner, as well as observing a cytotoxic effect and a significant increase in ROS production at 30 nM. The simultaneous treatment and the pre-treatment of HepG2 cells with red bean extract was not able to modify the cytotoxic T-2 effect. However, the simultaneous treatment of T-2 at 7.5 nM with the red bean extract showed a significant decrease in ROS production, with respect to the control. These results suggest that the red bean extract could modulate oxidative stress on HepG2 cells.
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spelling pubmed-89973702022-04-12 Effect of Phenolic Extract from Red Beans (Phaseolus vulgaris L.) on T-2 Toxin-Induced Cytotoxicity in HepG2 Cells Martínez-Alonso, Carmen Taroncher, Mercedes Castaldo, Luigi Izzo, Luana Rodríguez-Carrasco, Yelko Ritieni, Alberto Ruiz, María-José Foods Article Red beans contain human bioactive compounds such as polyphenols. Several in vitro studies have proposed the natural compounds as an innovative strategy to modify the toxic effects produced by mycotoxins. Hence, in this work, a complete investigation of the polyphenolic fraction of red beans was performed using a Q-Orbitrap high-resolution mass spectrometry analysis. Notably, epicatechin and delphinidin were the most detected polyphenols found in red bean extracts (3.297 and 3.108 mg/Kg, respectively). Moreover, the red bean extract was evaluated against the T-2 toxin (T-2) induced cytotoxicity in hepatocarcinoma cells (HepG2) by direct treatment, simultaneous treatment, and pre-treatment assays. These data showed that T-2 affected the cell viability in a dose-dependent manner, as well as observing a cytotoxic effect and a significant increase in ROS production at 30 nM. The simultaneous treatment and the pre-treatment of HepG2 cells with red bean extract was not able to modify the cytotoxic T-2 effect. However, the simultaneous treatment of T-2 at 7.5 nM with the red bean extract showed a significant decrease in ROS production, with respect to the control. These results suggest that the red bean extract could modulate oxidative stress on HepG2 cells. MDPI 2022-04-02 /pmc/articles/PMC8997370/ /pubmed/35407120 http://dx.doi.org/10.3390/foods11071033 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Martínez-Alonso, Carmen
Taroncher, Mercedes
Castaldo, Luigi
Izzo, Luana
Rodríguez-Carrasco, Yelko
Ritieni, Alberto
Ruiz, María-José
Effect of Phenolic Extract from Red Beans (Phaseolus vulgaris L.) on T-2 Toxin-Induced Cytotoxicity in HepG2 Cells
title Effect of Phenolic Extract from Red Beans (Phaseolus vulgaris L.) on T-2 Toxin-Induced Cytotoxicity in HepG2 Cells
title_full Effect of Phenolic Extract from Red Beans (Phaseolus vulgaris L.) on T-2 Toxin-Induced Cytotoxicity in HepG2 Cells
title_fullStr Effect of Phenolic Extract from Red Beans (Phaseolus vulgaris L.) on T-2 Toxin-Induced Cytotoxicity in HepG2 Cells
title_full_unstemmed Effect of Phenolic Extract from Red Beans (Phaseolus vulgaris L.) on T-2 Toxin-Induced Cytotoxicity in HepG2 Cells
title_short Effect of Phenolic Extract from Red Beans (Phaseolus vulgaris L.) on T-2 Toxin-Induced Cytotoxicity in HepG2 Cells
title_sort effect of phenolic extract from red beans (phaseolus vulgaris l.) on t-2 toxin-induced cytotoxicity in hepg2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997370/
https://www.ncbi.nlm.nih.gov/pubmed/35407120
http://dx.doi.org/10.3390/foods11071033
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