Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE)

One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed. Simultaneously, there are exponentially many choices for multiplex primer sequence selection, resulting in...

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Detalles Bibliográficos
Autores principales: Xie, Nina G., Wang, Michael X., Song, Ping, Mao, Shiqi, Wang, Yifan, Yang, Yuxia, Luo, Junfeng, Ren, Shengxiang, Zhang, David Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9001684/
https://www.ncbi.nlm.nih.gov/pubmed/35410464
http://dx.doi.org/10.1038/s41467-022-29500-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9001684/
https://www.ncbi.nlm.nih.gov/pubmed/35410464
http://dx.doi.org/10.1038/s41467-022-29500-4

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