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NMR Assignment of Methyl Groups in Immobilized Proteins Using Multiple-Bond (13)C Homonuclear Transfers, Proton Detection, and Very Fast MAS

In nuclear magnetic resonance spectroscopy of proteins, methyl protons play a particular role as extremely sensitive reporters on dynamics, allosteric effects, and protein–protein interactions, accessible even in high-molecular-weight systems approaching 1 MDa. The notorious issue of their chemical...

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Detalles Bibliográficos
Autores principales: Paluch, Piotr, Augustyniak, Rafal, Org, Mai-Liis, Vanatalu, Kalju, Kaldma, Ats, Samoson, Ago, Stanek, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9002630/
https://www.ncbi.nlm.nih.gov/pubmed/35425812
http://dx.doi.org/10.3389/fmolb.2022.828785