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NMR Assignment of Methyl Groups in Immobilized Proteins Using Multiple-Bond (13)C Homonuclear Transfers, Proton Detection, and Very Fast MAS
In nuclear magnetic resonance spectroscopy of proteins, methyl protons play a particular role as extremely sensitive reporters on dynamics, allosteric effects, and protein–protein interactions, accessible even in high-molecular-weight systems approaching 1 MDa. The notorious issue of their chemical...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9002630/ https://www.ncbi.nlm.nih.gov/pubmed/35425812 http://dx.doi.org/10.3389/fmolb.2022.828785 |