Cargando…

Targeted Elimination of bla(NDM-5) Gene in Escherichia coli by Conjugative CRISPR-Cas9 System

PURPOSE: Plasmid-borne carbapenem resistance gene bla(NDM-5) accelerates the dissemination of carbapenem-resistant Enterobacteriaceae. To efficiently eliminate the bla(NDM-5)-harboring plasmid and sensitize the antibiotic-resistant bacteria to meropenem, we used the CRISPR-Cas9 system for combating...

Descripción completa

Detalles Bibliográficos
Autores principales:	Li, Peisi, Wan, Peng, Zhao, Ruonan, Chen, Jin, Li, Xiaoshen, Li, Jie, Xiong, Wenguang, Zeng, Zhenling
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Dove 2022
Materias:	Original Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004731/ https://www.ncbi.nlm.nih.gov/pubmed/35422639 http://dx.doi.org/10.2147/IDR.S357470

Ejemplares similares

Reversal of mcr-1-Mediated Colistin Resistance in Escherichia coli by CRISPR-Cas9 System
por: Wan, Peng, et al.
Publicado: (2020)

Elimination of bla(KPC−2)-mediated carbapenem resistance in Escherichia coli by CRISPR-Cas9 system
por: Tao, Shuan, et al.
Publicado: (2023)

Genetic Diversity and Characteristics of bla(NDM)-Positive Plasmids in Escherichia coli
por: Zhang, Zhiren, et al.
Publicado: (2021)

Detection of NDM Variants (bla(NDM-1), bla(NDM-2), bla(NDM-3)) from Carbapenem-Resistant Escherichia coli and Klebsiella pneumoniae: First Report from Nepal
por: Thapa, Anisha, et al.
Publicado: (2022)

Identification of a Novel bla(NDM) Variant, bla(NDM-33,) in an Escherichia coli Isolate from Hospital Wastewater in China
por: Wang, Tao, et al.
Publicado: (2021)

Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine Escherichia coli Isolates in Guangdong, China
por: Ma, Zhenbao, et al.
Publicado: (2021)

Complete Nucleotide Sequence of Plasmids of Two Escherichia coli Strains Carrying bla(NDM–)(5) and bla(NDM)(–)(5) and bla(OXA)(–)(181) From the Same Patient
por: Marchetti, Vittoria Mattioni, et al.
Publicado: (2020)

Synergistic Antibacterial Effects of Meropenem in Combination with Aminoglycosides against Carbapenem-Resistant Escherichia coli Harboring bla(NDM-1) and bla(NDM-5)
por: Terbtothakun, Pawarisa, et al.
Publicado: (2021)

First identification of coexistence of bla(NDM-1) and bla(CMY-42) among Escherichia coli ST167 clinical isolates
por: Zhang, Xueqing, et al.
Publicado: (2013)

Prevalence and Distribution Characteristics of bla(KPC-2) and bla(NDM-1) Genes in Klebsiella pneumoniae
por: Zhang, Xiufeng, et al.
Publicado: (2020)

Decreased Fitness and Virulence in ST10 Escherichia coli Harboring bla(NDM-5) and mcr-1 against a ST4981 Strain with bla(NDM-5)
por: Zhang, Yawei, et al.
Publicado: (2017)

Escherichia coli of sequence type 3835 carrying bla(NDM-1), bla(CTX-M-15), bla(CMY-42) and bla(SHV-12)
por: Feng, Yu, et al.
Publicado: (2015)

Cryptic transmission of ST405 Escherichia coli carrying bla(NDM-4) in hospital
por: Zhang, Xiaoxia, et al.
Publicado: (2018)

Whole-Genome Analysis of bla(NDM)-Bearing Proteus mirabilis Isolates and mcr-1-Positive Escherichia coli Isolates Carrying bla(NDM) from the Same Fresh Vegetables in China
por: Li, Chang-An, et al.
Publicado: (2023)

Characterization of bla(NDM-5)-and bla(CTX-M-199)-Producing ST167 Escherichia coli Isolated from Shared Bikes
por: Chen, Qiyan, et al.
Publicado: (2022)

Molecular epidemiology and transmission of rmtB-positive Escherichia coli among ducks and environment
por: Li, Guihua, et al.
Publicado: (2023)

In Silico Characterization of bla(NDM)-Harboring Conjugative Plasmids in Acinetobacter Species
por: Tang, Biao, et al.
Publicado: (2022)

Genetic and enzymatic characterization of two novel bla(NDM-36, -37) variants in Escherichia coli strains
por: Ma, Wanshan, et al.
Publicado: (2023)

IS26 Is Responsible for the Evolution and Transmission of bla(NDM)-Harboring Plasmids in Escherichia coli of Poultry Origin in China
por: Zhao, Qiu-Yun, et al.
Publicado: (2021)

Phylogenetically Diverse Escherichia coli Strains from Chicken Coharbor Multiple Carbapenemase-Encoding Genes (bla(NDM)-bla(OXA)-blaI(MP))
por: Aklilu, Erkihun, et al.
Publicado: (2021)

Genetic characterization of bla(NDM)-harboring plasmids in carbapenem-resistant Escherichia coli from Myanmar
por: Sugawara, Yo, et al.
Publicado: (2017)

Role of Chromosome- and/or Plasmid-Located bla(NDM) on the Carbapenem Resistance and the Gene Stability in Escherichia coli
por: Sakamoto, Noriko, et al.
Publicado: (2022)

Draft Genome Sequence of NDM-5-Producing Escherichia coli Sequence Type 648 and Genetic Context of bla(NDM-5) in Australia
por: Wailan, Alexander M., et al.
Publicado: (2015)

Molecular Epidemiology of mcr-1, bla(KPC-2,) and bla(NDM-1) Harboring Clinically Isolated Escherichia coli from Pakistan
por: Bilal, Hazrat, et al.
Publicado: (2021)

High Prevalence and Diversity Characteristics of bla (NDM), mcr, and bla (ESBLs) Harboring Multidrug-Resistant Escherichia coli From Chicken, Pig, and Cattle in China
por: Liu, Zhihai, et al.
Publicado: (2022)

Correction: Genetic characterization of bla(NDM)-harboring plasmids in carbapenem-resistant Escherichia coli from Myanmar
Publicado: (2017)

Host dependent maintenance of a bla(NDM-1)-encoding plasmid in clinical Escherichia coli isolates
por: Gama, João Alves, et al.
Publicado: (2020)

Characterization Of bla(NDM-5)-Positive Escherichia coli Prevalent In A University Hospital In Eastern China
por: Sun, Pengfei, et al.
Publicado: (2019)

Characterization of NDM-5 Carbapenemase-Encoding Gene (bla(NDM-5)) – Positive Multidrug Resistant Commensal Escherichia coli from Diarrheal Patients
por: Chowdhury, Goutam, et al.
Publicado: (2022)

Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9
por: Zhao, Dongdong, et al.
Publicado: (2016)

CRISPR-Cas adaptation in Escherichia coli
por: Mitić, Damjan, et al.
Publicado: (2023)

Emergence of NDM-5-Producing Escherichia coli in a Teaching Hospital in Chongqing, China: IncF-Type Plasmids May Contribute to the Prevalence of bla(NDM–)(5)
por: Zou, Hua, et al.
Publicado: (2020)

A Natural Novel Mutation in the bla(NDM-5) Promoter Reducing Carbapenems Resistance in a Clinical Escherichia coli Strain
por: Li, Yiming, et al.
Publicado: (2022)

Polymyxin Combinations Combat Escherichia coli Harboring mcr-1 and bla(NDM-5): Preparation for a Postantibiotic Era
por: Bulman, Zackery P., et al.
Publicado: (2017)

Genetic characteristic of coexisting of mcr-1 and bla(NDM-5) in Escherichia coli isolates from lesion-bearing animal organs
por: Xiang, Yungai, et al.
Publicado: (2023)

High Prevalence of bla(NDM) Variants Among Carbapenem-Resistant Escherichia coli in Northern Jiangsu Province, China
por: Bi, Ruru, et al.
Publicado: (2018)

CRISPR/Cas9-mediated targeted chromosome elimination
por: Zuo, Erwei, et al.
Publicado: (2017)

Structural Diversity, Fitness Cost, and Stability of a Bla(NDM-1)-Bearing Cointegrate Plasmid in Klebsiella pneumoniae and Escherichia coli
por: Liu, Ziyi, et al.
Publicado: (2021)

Coexistence of tet(X4), mcr-1, and bla(NDM-5) in ST6775 Escherichia coli Isolates of Animal Origin in China
por: Lu, Xiaoyu, et al.
Publicado: (2022)

Report and Comparative Genomics of an NDM-5-Producing Escherichia coli in a Portuguese Hospital: Complex Class 1 Integrons as Important Players in bla(NDM) Spread
por: Tavares, Rafael D. S., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_0le3jmeuvdf04j96ntvpfvbq9r