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Fast and flexible processing of large FRET image stacks using the FRET-IBRA toolkit

Ratiometric time-lapse FRET analysis requires a robust and accurate processing pipeline to eliminate bias in intensity measurements on fluorescent images before further quantitative analysis can be conducted. This level of robustness can only be achieved by supplementing automated tools with built-i...

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Detalles Bibliográficos
Autores principales: Munglani, Gautam, Vogler, Hannes, Grossniklaus, Ueli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009774/
https://www.ncbi.nlm.nih.gov/pubmed/35377870
http://dx.doi.org/10.1371/journal.pcbi.1009242