PABLO-QA: A sensitive assay for quantifying monophosphorylated RNA 5′ ends

Generated by RNA deprotection or cleavage, 5′ monophosphates trigger RNA degradation in all organisms. Here we describe PABLO-QA (Phosphorylation Assay By Ligation of Oligonucleotides and Quantitative Amplification), a sensitive, low-cost procedure for determining the percentage of specific RNA 5′ e...

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Detalles Bibliográficos
Autores principales: Richards, Jamie, Belasco, Joel G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010794/
https://www.ncbi.nlm.nih.gov/pubmed/35434657
http://dx.doi.org/10.1016/j.xpro.2022.101190

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010794/
https://www.ncbi.nlm.nih.gov/pubmed/35434657
http://dx.doi.org/10.1016/j.xpro.2022.101190

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