Characterization of antioxidant active biopolymer bilayer film based on gelatin-frankincense incorporated with ascorbic acid and Hyssopus officinalis essential oil

In this study, a bio-based bilayer edible film based on gelatin/frankincense, with the incorporation of different concentrations of Ascorbic acid (AA) (0, 1, 2%) into the inner layer (gelatin) and Hyssopus officinalis (HO) (0, 0.75, 1.5%) essential oil in the outer layer (frankincense) was prepared. A significant increase (p < 0.05) in b* and a remarkable decrease in whiteness and lightness of the films were seen via increasing the HO ascribed to the Total Phenolic Content of HO and non-enzymatic browning. Although there was a significant decrease (p < 0.05) in Tensile Strength with the addition of HO, Elongation at Break was increased significantly as a function of HO, which is correlated with a dense and compact network in SEM images. The maximum thickness of film emulsified with 1.5%HO + 2%AA ascribed to the accumulation of solid content. The improvement in Water Contact Angle (℃) and a reduction in Water Vapor Permeability (gr/s mPa) have occurred due to the hydrophobic nature of HO.