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Metabolomics Analysis of Morchella sp. From Different Geographical Origins of China Using UPLC-Q-TOF-MS

The morel mushroom (Morchella sp.) is reputed as one of the most highly-prized edible fungi with mounting cultivated area as well as commercial popularity in China. To date, optimized methods specific for quality evaluation and constituent analysis of Morchella sp. are still non-available, impeding...

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Autores principales: Dong, Hui, Zhao, Xiaoyan, Cai, Min, Gu, Haotian, E, Hengchao, Li, Xiaobei, Zhang, Yanmei, Lu, Huan, Zhou, Changyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016275/
https://www.ncbi.nlm.nih.gov/pubmed/35449541
http://dx.doi.org/10.3389/fnut.2022.865531
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author Dong, Hui
Zhao, Xiaoyan
Cai, Min
Gu, Haotian
E, Hengchao
Li, Xiaobei
Zhang, Yanmei
Lu, Huan
Zhou, Changyan
author_facet Dong, Hui
Zhao, Xiaoyan
Cai, Min
Gu, Haotian
E, Hengchao
Li, Xiaobei
Zhang, Yanmei
Lu, Huan
Zhou, Changyan
author_sort Dong, Hui
collection PubMed
description The morel mushroom (Morchella sp.) is reputed as one of the most highly-prized edible fungi with mounting cultivated area as well as commercial popularity in China. To date, optimized methods specific for quality evaluation and constituent analysis of Morchella sp. are still non-available, impeding the healthy and sustainable development of this industry. Herein, an untargeted UPLC-Q-TOF-MS-based metabolomics approach was performed to characterize the metabolite profiles of morel samples from four distinct geographical origins of China, viz. Gansu, Guizhou, Liaoning, and Henan province. A total of 32 significantly different metabolites assigned to lipids (19), organic acids (9), amino acids (3), and ketones (1) were identified to distinguish the geographic-segregation samples amenable to multivariate analysis. These metabolites may serve as molecular markers indicative of specific regions. More importantly, the lipid, protein and amino acid metabolism were responsible for geographic differences as revealed by KEGG pathway enrichment analysis. Collectively, this study not only pioneered high-throughput methodology to evaluate quality of Morchella sp. and distinguish geographical origins in a sensitive, rapid and efficient manner, but also shed light on the potential link between physiochemical variation and geological origins from a metabolic perspective, which may be conducive to the advancement of edible fungi industry and establishment of food traceability system.
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spelling pubmed-90162752022-04-20 Metabolomics Analysis of Morchella sp. From Different Geographical Origins of China Using UPLC-Q-TOF-MS Dong, Hui Zhao, Xiaoyan Cai, Min Gu, Haotian E, Hengchao Li, Xiaobei Zhang, Yanmei Lu, Huan Zhou, Changyan Front Nutr Nutrition The morel mushroom (Morchella sp.) is reputed as one of the most highly-prized edible fungi with mounting cultivated area as well as commercial popularity in China. To date, optimized methods specific for quality evaluation and constituent analysis of Morchella sp. are still non-available, impeding the healthy and sustainable development of this industry. Herein, an untargeted UPLC-Q-TOF-MS-based metabolomics approach was performed to characterize the metabolite profiles of morel samples from four distinct geographical origins of China, viz. Gansu, Guizhou, Liaoning, and Henan province. A total of 32 significantly different metabolites assigned to lipids (19), organic acids (9), amino acids (3), and ketones (1) were identified to distinguish the geographic-segregation samples amenable to multivariate analysis. These metabolites may serve as molecular markers indicative of specific regions. More importantly, the lipid, protein and amino acid metabolism were responsible for geographic differences as revealed by KEGG pathway enrichment analysis. Collectively, this study not only pioneered high-throughput methodology to evaluate quality of Morchella sp. and distinguish geographical origins in a sensitive, rapid and efficient manner, but also shed light on the potential link between physiochemical variation and geological origins from a metabolic perspective, which may be conducive to the advancement of edible fungi industry and establishment of food traceability system. Frontiers Media S.A. 2022-04-05 /pmc/articles/PMC9016275/ /pubmed/35449541 http://dx.doi.org/10.3389/fnut.2022.865531 Text en Copyright © 2022 Dong, Zhao, Cai, Gu, E, Li, Zhang, Lu and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Dong, Hui
Zhao, Xiaoyan
Cai, Min
Gu, Haotian
E, Hengchao
Li, Xiaobei
Zhang, Yanmei
Lu, Huan
Zhou, Changyan
Metabolomics Analysis of Morchella sp. From Different Geographical Origins of China Using UPLC-Q-TOF-MS
title Metabolomics Analysis of Morchella sp. From Different Geographical Origins of China Using UPLC-Q-TOF-MS
title_full Metabolomics Analysis of Morchella sp. From Different Geographical Origins of China Using UPLC-Q-TOF-MS
title_fullStr Metabolomics Analysis of Morchella sp. From Different Geographical Origins of China Using UPLC-Q-TOF-MS
title_full_unstemmed Metabolomics Analysis of Morchella sp. From Different Geographical Origins of China Using UPLC-Q-TOF-MS
title_short Metabolomics Analysis of Morchella sp. From Different Geographical Origins of China Using UPLC-Q-TOF-MS
title_sort metabolomics analysis of morchella sp. from different geographical origins of china using uplc-q-tof-ms
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016275/
https://www.ncbi.nlm.nih.gov/pubmed/35449541
http://dx.doi.org/10.3389/fnut.2022.865531
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