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Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions

We report biocompatible materials for cryostorage of unstable proteins such as cancer-related enzyme, N-acetylglucosaminyltransferase-V (GnT-V). GnT-V activity and the amount of protein after freezing were better retained in synthetic zwitterion solutions than in the glycerol solution. This study hi...

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Detalles Bibliográficos
Autores principales: Hirata, Tetsuya, Takekiyo, Takahiro, Yoshimura, Yukihiro, Tokoro, Yuko, Ishizaki, Takeru, Kizuka, Yasuhiko, Kuroda, Kosuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016413/
https://www.ncbi.nlm.nih.gov/pubmed/35481097
http://dx.doi.org/10.1039/d2ra01575g