Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions

We report biocompatible materials for cryostorage of unstable proteins such as cancer-related enzyme, N-acetylglucosaminyltransferase-V (GnT-V). GnT-V activity and the amount of protein after freezing were better retained in synthetic zwitterion solutions than in the glycerol solution. This study hi...

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Detalles Bibliográficos
Autores principales: Hirata, Tetsuya, Takekiyo, Takahiro, Yoshimura, Yukihiro, Tokoro, Yuko, Ishizaki, Takeru, Kizuka, Yasuhiko, Kuroda, Kosuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016413/
https://www.ncbi.nlm.nih.gov/pubmed/35481097
http://dx.doi.org/10.1039/d2ra01575g
Descripción
Sumario:We report biocompatible materials for cryostorage of unstable proteins such as cancer-related enzyme, N-acetylglucosaminyltransferase-V (GnT-V). GnT-V activity and the amount of protein after freezing were better retained in synthetic zwitterion solutions than in the glycerol solution. This study highlights the potential utility of synthetic zwitterions as novel cryoprotectants.

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