Fluorescent labeling of genomic loci in Drosophila imaginal discs with heterologous DNA-binding proteins

Using the Drosophila melanogaster Hox gene Ultrabithorax (Ubx) as an example, we demonstrate the use of three heterologous DNA-binding protein systems—LacI/LacO, ParB1/ParS1, and ParB2/ParS2—to label genomic loci in imaginal discs with the insertion of a small DNA tag. We compare each system, consid...

Descripción completa

Detalles Bibliográficos
Autores principales: Delker, Rebecca K., Munce, Ross H., Hu, Michelle, Mann, Richard S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017127/
https://www.ncbi.nlm.nih.gov/pubmed/35475221
http://dx.doi.org/10.1016/j.crmeth.2022.100175

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017127/
https://www.ncbi.nlm.nih.gov/pubmed/35475221
http://dx.doi.org/10.1016/j.crmeth.2022.100175

Ejemplares similares