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Fluorescent labeling of genomic loci in Drosophila imaginal discs with heterologous DNA-binding proteins
Using the Drosophila melanogaster Hox gene Ultrabithorax (Ubx) as an example, we demonstrate the use of three heterologous DNA-binding protein systems—LacI/LacO, ParB1/ParS1, and ParB2/ParS2—to label genomic loci in imaginal discs with the insertion of a small DNA tag. We compare each system, consid...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017127/ https://www.ncbi.nlm.nih.gov/pubmed/35475221 http://dx.doi.org/10.1016/j.crmeth.2022.100175 |