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Four-color single-molecule imaging with engineered tags resolves the molecular architecture of signaling complexes in the plasma membrane

Localization and tracking of individual receptors by single-molecule imaging opens unique possibilities to unravel the assembly and dynamics of signaling complexes in the plasma membrane. We present a comprehensive workflow for imaging and analyzing receptor diffusion and interaction in live cells a...

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Detalles Bibliográficos
Autores principales: Sotolongo Bellón, Junel, Birkholz, Oliver, Richter, Christian P., Eull, Florian, Kenneweg, Hella, Wilmes, Stephan, Rothbauer, Ulrich, You, Changjiang, Walter, Mark R., Kurre, Rainer, Piehler, Jacob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017138/
https://www.ncbi.nlm.nih.gov/pubmed/35474965
http://dx.doi.org/10.1016/j.crmeth.2022.100165