Principles and pitfalls of high-throughput analysis of microRNA-binding thermodynamics and kinetics by RNA Bind-n-Seq

RNA Bind-n-Seq (RBNS) is a cost-effective, high-throughput method capable of identifying the sequence preferences of RNA-binding proteins and of qualitatively defining relative dissociation constants. Although RBNS is often described as an unbiased method, several factors may influence the outcome o...

Descripción completa

Detalles Bibliográficos
Autores principales: Jouravleva, Karina, Vega-Badillo, Joel, Zamore, Phillip D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017153/
https://www.ncbi.nlm.nih.gov/pubmed/35475222
http://dx.doi.org/10.1016/j.crmeth.2022.100185

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017153/
https://www.ncbi.nlm.nih.gov/pubmed/35475222
http://dx.doi.org/10.1016/j.crmeth.2022.100185

Ejemplares similares