Construction of Strand-seq libraries in open nanoliter arrays

Single-cell Strand-seq generates directional genomic information to study DNA repair, assemble genomes, and map structural variation onto chromosome-length haplotypes. We report a nanoliter-volume, one-pot (OP) Strand-seq library preparation protocol in which reagents are added cumulatively, DNA pur...

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Detalles Bibliográficos
Autores principales: Hanlon, Vincent C.T., Chan, Daniel D., Hamadeh, Zeid, Wang, Yanni, Mattsson, Carl-Adam, Spierings, Diana C.J., Coope, Robin J.N., Lansdorp, Peter M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017222/
https://www.ncbi.nlm.nih.gov/pubmed/35474869
http://dx.doi.org/10.1016/j.crmeth.2021.100150

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017222/
https://www.ncbi.nlm.nih.gov/pubmed/35474869
http://dx.doi.org/10.1016/j.crmeth.2021.100150

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