Construction of Strand-seq libraries in open nanoliter arrays

Single-cell Strand-seq generates directional genomic information to study DNA repair, assemble genomes, and map structural variation onto chromosome-length haplotypes. We report a nanoliter-volume, one-pot (OP) Strand-seq library preparation protocol in which reagents are added cumulatively, DNA pur...

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Detalles Bibliográficos
Autores principales: Hanlon, Vincent C.T., Chan, Daniel D., Hamadeh, Zeid, Wang, Yanni, Mattsson, Carl-Adam, Spierings, Diana C.J., Coope, Robin J.N., Lansdorp, Peter M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017222/
https://www.ncbi.nlm.nih.gov/pubmed/35474869
http://dx.doi.org/10.1016/j.crmeth.2021.100150
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author Hanlon, Vincent C.T.
Chan, Daniel D.
Hamadeh, Zeid
Wang, Yanni
Mattsson, Carl-Adam
Spierings, Diana C.J.
Coope, Robin J.N.
Lansdorp, Peter M.
author_facet Hanlon, Vincent C.T.
Chan, Daniel D.
Hamadeh, Zeid
Wang, Yanni
Mattsson, Carl-Adam
Spierings, Diana C.J.
Coope, Robin J.N.
Lansdorp, Peter M.
author_sort Hanlon, Vincent C.T.
collection PubMed
description Single-cell Strand-seq generates directional genomic information to study DNA repair, assemble genomes, and map structural variation onto chromosome-length haplotypes. We report a nanoliter-volume, one-pot (OP) Strand-seq library preparation protocol in which reagents are added cumulatively, DNA purification steps are avoided, and enzymes are inactivated with a thermolabile protease. OP-Strand-seq libraries capture 10%–25% of the genome from a single-cell with reduced costs and increased throughput.
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spelling pubmed-90172222022-04-25 Construction of Strand-seq libraries in open nanoliter arrays Hanlon, Vincent C.T. Chan, Daniel D. Hamadeh, Zeid Wang, Yanni Mattsson, Carl-Adam Spierings, Diana C.J. Coope, Robin J.N. Lansdorp, Peter M. Cell Rep Methods Report Single-cell Strand-seq generates directional genomic information to study DNA repair, assemble genomes, and map structural variation onto chromosome-length haplotypes. We report a nanoliter-volume, one-pot (OP) Strand-seq library preparation protocol in which reagents are added cumulatively, DNA purification steps are avoided, and enzymes are inactivated with a thermolabile protease. OP-Strand-seq libraries capture 10%–25% of the genome from a single-cell with reduced costs and increased throughput. Elsevier 2022-01-24 /pmc/articles/PMC9017222/ /pubmed/35474869 http://dx.doi.org/10.1016/j.crmeth.2021.100150 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Report
Hanlon, Vincent C.T.
Chan, Daniel D.
Hamadeh, Zeid
Wang, Yanni
Mattsson, Carl-Adam
Spierings, Diana C.J.
Coope, Robin J.N.
Lansdorp, Peter M.
Construction of Strand-seq libraries in open nanoliter arrays
title Construction of Strand-seq libraries in open nanoliter arrays
title_full Construction of Strand-seq libraries in open nanoliter arrays
title_fullStr Construction of Strand-seq libraries in open nanoliter arrays
title_full_unstemmed Construction of Strand-seq libraries in open nanoliter arrays
title_short Construction of Strand-seq libraries in open nanoliter arrays
title_sort construction of strand-seq libraries in open nanoliter arrays
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017222/
https://www.ncbi.nlm.nih.gov/pubmed/35474869
http://dx.doi.org/10.1016/j.crmeth.2021.100150
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