Secondary structure RNA elements control the cleavage activity of DICER

The accurate and efficient cleavage of shRNAs and pre-miRNAs by DICER is crucial for their gene-silencing activity. Here, we conduct high-throughput DICER cleavage assays for more than ~20,000 different shRNAs and show the comprehensive cleavage activities of DICER on these sequences. We discover a...

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Detalles Bibliográficos
Autores principales: Nguyen, Trung Duc, Trinh, Tam Anh, Bao, Sheng, Nguyen, Tuan Anh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9018771/
https://www.ncbi.nlm.nih.gov/pubmed/35440644
http://dx.doi.org/10.1038/s41467-022-29822-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9018771/
https://www.ncbi.nlm.nih.gov/pubmed/35440644
http://dx.doi.org/10.1038/s41467-022-29822-3

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