Nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos

Reprogramming of somatic nuclei toward the embryonic state has been studied using nuclear transfer (NT) to an oocyte at the metaphase II (MII) stage. In this NT, a somatic nucleus transplanted into an MII oocyte of the same species undergoes DNA replication and cell division before activating embryo...

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Detalles Bibliográficos
Autores principales: Tomikawa, Junko, Penfold, Christopher A., Hatakeyama, Rena, Miyamoto, Kei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019708/
https://www.ncbi.nlm.nih.gov/pubmed/35463476
http://dx.doi.org/10.1016/j.xpro.2022.101284
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author Tomikawa, Junko
Penfold, Christopher A.
Hatakeyama, Rena
Miyamoto, Kei
author_facet Tomikawa, Junko
Penfold, Christopher A.
Hatakeyama, Rena
Miyamoto, Kei
author_sort Tomikawa, Junko
collection PubMed
description Reprogramming of somatic nuclei toward the embryonic state has been studied using nuclear transfer (NT) to an oocyte at the metaphase II (MII) stage. In this NT, a somatic nucleus transplanted into an MII oocyte of the same species undergoes DNA replication and cell division before activating embryonic genes. Here, we describe a direct NT protocol using 4-cell stage mouse embryos that enables reprogramming of intra- and cross-species nuclei to express embryonic genes without requiring DNA replication and cell division. For complete details on the use and execution of this protocol, please refer to Tomikawa et al. (2021).
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spelling pubmed-90197082022-04-21 Nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos Tomikawa, Junko Penfold, Christopher A. Hatakeyama, Rena Miyamoto, Kei STAR Protoc Protocol Reprogramming of somatic nuclei toward the embryonic state has been studied using nuclear transfer (NT) to an oocyte at the metaphase II (MII) stage. In this NT, a somatic nucleus transplanted into an MII oocyte of the same species undergoes DNA replication and cell division before activating embryonic genes. Here, we describe a direct NT protocol using 4-cell stage mouse embryos that enables reprogramming of intra- and cross-species nuclei to express embryonic genes without requiring DNA replication and cell division. For complete details on the use and execution of this protocol, please refer to Tomikawa et al. (2021). Elsevier 2022-04-11 /pmc/articles/PMC9019708/ /pubmed/35463476 http://dx.doi.org/10.1016/j.xpro.2022.101284 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Tomikawa, Junko
Penfold, Christopher A.
Hatakeyama, Rena
Miyamoto, Kei
Nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos
title Nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos
title_full Nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos
title_fullStr Nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos
title_full_unstemmed Nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos
title_short Nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos
title_sort nuclear transfer system for the direct induction of embryonic transcripts from intra- and cross-species nuclei using mouse 4-cell embryos
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019708/
https://www.ncbi.nlm.nih.gov/pubmed/35463476
http://dx.doi.org/10.1016/j.xpro.2022.101284
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