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Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis

TaqMan probe-based commercial real-time (RT) PCR kits are expensive but most frequently used in COVID-19 diagnosis. The unprecedented scale of SARS-CoV-2 infections needs to meet the challenge of testing more persons at a reasonable cost. This study developed a simple and cost-effective alternative...

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Detalles Bibliográficos
Autores principales: Sarkar, Shovon Lal, Alam, A. S. M. Rubayet Ul, Das, Prosanto Kumar, Pramanik, Md. Hasan Ali, Al-Emran, Hassan M., Jahid, Iqbal Kabir, Hossain, M. Anwar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019801/
https://www.ncbi.nlm.nih.gov/pubmed/35444203
http://dx.doi.org/10.1038/s41598-022-10413-7