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Effectiveness of blocking primers and a peptide nucleic acid (PNA) clamp for 18S metabarcoding dietary analysis of herbivorous fish

The structure of food webs and carbon flow in aquatic ecosystems can be better understood by studying contributing factors such as the diets of herbivorous fish. Metabarcoding using a high-throughput sequencer has recently been used to clarify prey organisms of various fish except herbivorous fish....

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Detalles Bibliográficos
Autores principales: Homma, Chiho, Inokuchi, Daiki, Nakamura, Yohei, Uy, Wilfredo H., Ohnishi, Kouhei, Yamaguchi, Haruo, Adachi, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020718/
https://www.ncbi.nlm.nih.gov/pubmed/35442965
http://dx.doi.org/10.1371/journal.pone.0266268