Cargando…

Effectiveness of blocking primers and a peptide nucleic acid (PNA) clamp for 18S metabarcoding dietary analysis of herbivorous fish

The structure of food webs and carbon flow in aquatic ecosystems can be better understood by studying contributing factors such as the diets of herbivorous fish. Metabarcoding using a high-throughput sequencer has recently been used to clarify prey organisms of various fish except herbivorous fish....

Descripción completa

Detalles Bibliográficos
Autores principales:	Homma, Chiho, Inokuchi, Daiki, Nakamura, Yohei, Uy, Wilfredo H., Ohnishi, Kouhei, Yamaguchi, Haruo, Adachi, Masao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020718/ https://www.ncbi.nlm.nih.gov/pubmed/35442965 http://dx.doi.org/10.1371/journal.pone.0266268

Ejemplares similares

PNA Clamping in Nucleic Acid Amplification Protocols to Detect Single Nucleotide Mutations Related to Cancer
por: Fouz, Munira F., et al.
Publicado: (2020)

Detection of EGFR mutations in circulating free DNA by PNA-mediated PCR clamping
por: Kim, Hye-Ryoun, et al.
Publicado: (2013)

PNA-Pdx: Versatile Peptide Nucleic Acid-Based Detection of Nucleic Acids and SNPs
por: Jiang, Wenjun, et al.
Publicado: (2023)

Characteristics and prognostic analysis of patients with detected KRAS mutations in resected lung adenocarcinomas by peptide nucleic acid-locked nucleic acid polymerase chain reaction (PNA-LNA PCR) clamp method
por: Hashimoto, Tatsuya, et al.
Publicado: (2023)

Evaluation of plant contamination in metabarcoding diet analysis of a herbivore
por: Ando, Haruko, et al.
Publicado: (2018)

DISSECT Method Using PNA-LNA Clamp Improves Detection of EGFR T790m Mutation
por: Guha, Minakshi, et al.
Publicado: (2013)

Detection of activating and acquired resistant mutation in plasma from EGFR-mutated NSCLC patients by peptide nucleic acid (PNA) clamping-assisted fluorescence melting curve analysis
por: Kim, Chang Gon, et al.
Publicado: (2017)

Validation of COI metabarcoding primers for terrestrial arthropods
por: Elbrecht, Vasco, et al.
Publicado: (2019)

EGFR Mutation Analysis of Circulating Tumor DNA Using an Improved PNA-LNA PCR Clamp Method
por: Watanabe, Kana, et al.
Publicado: (2016)

Vitamin B(12) as a carrier of peptide nucleic acid (PNA) into bacterial cells
por: Równicki, Marcin, et al.
Publicado: (2017)

Cooperative Cellular Uptake and Activity of Octaarginine Antisense Peptide Nucleic Acid (PNA) Conjugates
por: Ghavami, Mahdi, et al.
Publicado: (2019)

Jumping the green wall: The use of PNA‐DNA clamps to enhance microbiome sampling depth in wildlife microbiome research
por: Víquez‐R, Luis, et al.
Publicado: (2020)

Application of Locked Nucleic Acid (LNA) Primer and PCR Clamping by LNA Oligonucleotide to Enhance the Amplification of Internal Transcribed Spacer (ITS) Regions in Investigating the Community Structures of Plant–Associated Fungi
por: Ikenaga, Makoto, et al.
Publicado: (2016)

Improved Synthesis Strategy for Peptide Nucleic Acids (PNA) appropriate for Cell-specific Fluorescence Imaging
por: Pipkorn, Rüdiger, et al.
Publicado: (2011)

Perspectives on conformationally constrained peptide nucleic acid (PNA): insights into the structural design, properties and applications
por: Suparpprom, Chaturong, et al.
Publicado: (2022)

Diet of a rare herbivore based on DNA metabarcoding of feces: Selection, seasonality, and survival
por: Goldberg, Amanda R., et al.
Publicado: (2020)

Gemdimethyl Peptide Nucleic Acids (α/β/γ-gdm-PNA): E/Z-Rotamers Influence the Selectivity in the Formation of Parallel/Antiparallel gdm-PNA:DNA/RNA Duplexes
por: Kulkarni, Pradnya, et al.
Publicado: (2022)

Fluorogenic PNA probes
por: Vilaivan, Tirayut
Publicado: (2018)

A Novel, Highly Selective RT-QPCR Method for Quantification of MSRV Using PNA Clamping Syncytin-1 (ERVWE1)
por: Machnik, Grzegorz, et al.
Publicado: (2015)

Development of a Detection System for ESR1 Mutations in Circulating Tumour DNA Using PNA-LNA-Mediated PCR Clamping
por: Kojima, Yuki, et al.
Publicado: (2023)

Discriminating Multi-Species Populations in Biofilms with Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA FISH)
por: Almeida, Carina, et al.
Publicado: (2011)

Side chain modified peptide nucleic acids (PNA) for knock-down of six3 in medaka embryos
por: Dorn, Sebastian, et al.
Publicado: (2012)

The first crystal structures of RNA–PNA duplexes and a PNA-PNA duplex containing mismatches—toward anti-sense therapy against TREDs
por: Kiliszek, Agnieszka, et al.
Publicado: (2016)

Strong primer bias for Tulasnellaceae fungi in metabarcoding: Specific primers improve the characterization of the mycorrhizal communities of epiphytic orchids
por: Rammitsu, Kento, et al.
Publicado: (2021)

Detection of Ki-ras mutations in tissue and plasma samples of patients with pancreatic cancer using PNA-mediated PCR clamping and hybridisation probes
por: Däbritz, J, et al.
Publicado: (2005)

Detection of K-Ras mutations in tumour samples of patients with non-small cell lung cancer using PNA-mediated PCR clamping
por: Beau-Faller, M, et al.
Publicado: (2009)

Detection of SF3B1 p.Lys700Glu Mutation by PNA-PCR Clamping in Myelodysplastic Syndromes and Myeloproliferative Neoplasms
por: Petiti, Jessica, et al.
Publicado: (2022)

Inhibition of Multidrug Resistance by SV40 Pseudovirion Delivery of an Antigene Peptide Nucleic Acid (PNA) in Cultured Cells
por: Macadangdang, Benjamin, et al.
Publicado: (2011)

Correction: Discriminating Multi-Species Populations in Biofilms with Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA FISH)
por: Almeida, Carina, et al.
Publicado: (2013)

Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria
por: Rocha, Rui, et al.
Publicado: (2018)

Comparison and Validation of Some ITS Primer Pairs Useful for Fungal Metabarcoding Studies
por: Op De Beeck, Michiel, et al.
Publicado: (2014)

New mitochondrial primers for metabarcoding of insects, designed and evaluated using in silico methods
por: Marquina, Daniel, et al.
Publicado: (2018)

In silico and empirical evaluation of twelve metabarcoding primer sets for insectivorous diet analyses
por: Tournayre, Orianne, et al.
Publicado: (2020)

New 12S metabarcoding primers for enhanced Neotropical freshwater fish biodiversity assessment
por: Milan, David T., et al.
Publicado: (2020)

Environmental DNA metabarcoding primers for freshwater fish detection and quantification: In silico and in tanks
por: Shu, Lu, et al.
Publicado: (2021)

Metabarcoding of Fecal Samples to Determine Herbivore Diets: A Case Study of the Endangered Pacific Pocket Mouse
por: Iwanowicz, Deborah D., et al.
Publicado: (2016)

Revealing cryptic interactions between large mammalian herbivores and plant‐dwelling arthropods via DNA metabarcoding
por: Berman, Tali S., et al.
Publicado: (2021)

Synthesis of PNA Oligoether Conjugates
por: Ghidini, Alice, et al.
Publicado: (2014)

Selective recognition of DNA from olive leaves and olive oil by PNA and modified-PNA microarrays
por: Rossi, Stefano, et al.
Publicado: (2012)

Subnanomolar antisense activity of phosphonate-peptide nucleic acid (PNA) conjugates delivered by cationic lipids to HeLa cells
por: Shiraishi, Takehiko, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_gt7q6dbvrfc4nirmm575l7q9ed