Organocatalyst treatment improves variant calling and mutant detection in archival clinical samples

Formalin fixation of biological specimens damages nucleic acids and limits their use in genomic analyses. Previously, we showed that RNA isolation with an organocatalyst (2-amino-5-methylphenyl phosphonic acid, used to speed up reversal of formalin-induced adducts) and extended heated incubation (OR...

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Detalles Bibliográficos
Autores principales: Wehmas, Leah C., Wood, Charles E., Guan, Ping, Gosink, Mark, Hester, Susan D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021284/
https://www.ncbi.nlm.nih.gov/pubmed/35443772
http://dx.doi.org/10.1038/s41598-022-10301-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021284/
https://www.ncbi.nlm.nih.gov/pubmed/35443772
http://dx.doi.org/10.1038/s41598-022-10301-0

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