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Effect of pH and Salinity on the Ability of Salmonella Serotypes to Form Biofilm

Salmonella is a major cause of food-borne infections in Europe, and the majority of human infections are caused by only a few serotypes, among them are Salmonella enterica subsp. enterica serotype Enteritidis (hereafter Salmonella Enteritidis), Salmonella Typhimurium, and the monophasic variant of S...

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Autores principales: Petrin, Sara, Mancin, Marzia, Losasso, Carmen, Deotto, Silvia, Olsen, John Elmerdahl, Barco, Lisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021792/
https://www.ncbi.nlm.nih.gov/pubmed/35464965
http://dx.doi.org/10.3389/fmicb.2022.821679
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author Petrin, Sara
Mancin, Marzia
Losasso, Carmen
Deotto, Silvia
Olsen, John Elmerdahl
Barco, Lisa
author_facet Petrin, Sara
Mancin, Marzia
Losasso, Carmen
Deotto, Silvia
Olsen, John Elmerdahl
Barco, Lisa
author_sort Petrin, Sara
collection PubMed
description Salmonella is a major cause of food-borne infections in Europe, and the majority of human infections are caused by only a few serotypes, among them are Salmonella enterica subsp. enterica serotype Enteritidis (hereafter Salmonella Enteritidis), Salmonella Typhimurium, and the monophasic variant of S. Typhimurium. The reason for this is not fully understood, but could include virulence factors as well as increased ability to transfer via the external environment. Formation of biofilm is considered an adaptation strategy used by bacteria to overcome environmental stresses. In order to assess the capability of different Salmonella serotypes to produce biofilm and establish whether this is affected by pH and salinity, 88 Salmonella isolates collected from animal, food, and human sources and belonging to 15 serotypes, including those most frequently responsible for human infections, were tested. Strains were grown in tryptic soy broth (TSB), TSB with 4% NaCl pH 4.5, TSB with 10% NaCl pH 4.5, TSB with 4% NaCl pH 7, or TSB with 10% NaCl pH 7, and biofilm production was assessed after 24 h at 37°C using crystal violet staining. A linear mixed effect model was applied to compare results from the different experimental conditions. Among the tested serotypes, S. Dublin showed the greatest ability to form biofilm even at pH 4.5, which inhibited biofilm production in the other tested serotypes. Salmonella Senftenberg and the monophasic variant of S. Typhimurium showed the highest biofilm production in TSB with 10% NaCl pH 7. In general, pH had a high influence on the ability to form biofilm, and most of the tested strains were not able to produce biofilm at pH 4.5. In contrast, salinity only had a limited influence on biofilm production. In general, serotypes causing the highest number of human infections showed a limited ability to produce biofilm in the tested conditions, indicating that biofilm formation is not a crucial factor in the success of these clones.
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spelling pubmed-90217922022-04-22 Effect of pH and Salinity on the Ability of Salmonella Serotypes to Form Biofilm Petrin, Sara Mancin, Marzia Losasso, Carmen Deotto, Silvia Olsen, John Elmerdahl Barco, Lisa Front Microbiol Microbiology Salmonella is a major cause of food-borne infections in Europe, and the majority of human infections are caused by only a few serotypes, among them are Salmonella enterica subsp. enterica serotype Enteritidis (hereafter Salmonella Enteritidis), Salmonella Typhimurium, and the monophasic variant of S. Typhimurium. The reason for this is not fully understood, but could include virulence factors as well as increased ability to transfer via the external environment. Formation of biofilm is considered an adaptation strategy used by bacteria to overcome environmental stresses. In order to assess the capability of different Salmonella serotypes to produce biofilm and establish whether this is affected by pH and salinity, 88 Salmonella isolates collected from animal, food, and human sources and belonging to 15 serotypes, including those most frequently responsible for human infections, were tested. Strains were grown in tryptic soy broth (TSB), TSB with 4% NaCl pH 4.5, TSB with 10% NaCl pH 4.5, TSB with 4% NaCl pH 7, or TSB with 10% NaCl pH 7, and biofilm production was assessed after 24 h at 37°C using crystal violet staining. A linear mixed effect model was applied to compare results from the different experimental conditions. Among the tested serotypes, S. Dublin showed the greatest ability to form biofilm even at pH 4.5, which inhibited biofilm production in the other tested serotypes. Salmonella Senftenberg and the monophasic variant of S. Typhimurium showed the highest biofilm production in TSB with 10% NaCl pH 7. In general, pH had a high influence on the ability to form biofilm, and most of the tested strains were not able to produce biofilm at pH 4.5. In contrast, salinity only had a limited influence on biofilm production. In general, serotypes causing the highest number of human infections showed a limited ability to produce biofilm in the tested conditions, indicating that biofilm formation is not a crucial factor in the success of these clones. Frontiers Media S.A. 2022-04-07 /pmc/articles/PMC9021792/ /pubmed/35464965 http://dx.doi.org/10.3389/fmicb.2022.821679 Text en Copyright © 2022 Petrin, Mancin, Losasso, Deotto, Olsen and Barco. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Petrin, Sara
Mancin, Marzia
Losasso, Carmen
Deotto, Silvia
Olsen, John Elmerdahl
Barco, Lisa
Effect of pH and Salinity on the Ability of Salmonella Serotypes to Form Biofilm
title Effect of pH and Salinity on the Ability of Salmonella Serotypes to Form Biofilm
title_full Effect of pH and Salinity on the Ability of Salmonella Serotypes to Form Biofilm
title_fullStr Effect of pH and Salinity on the Ability of Salmonella Serotypes to Form Biofilm
title_full_unstemmed Effect of pH and Salinity on the Ability of Salmonella Serotypes to Form Biofilm
title_short Effect of pH and Salinity on the Ability of Salmonella Serotypes to Form Biofilm
title_sort effect of ph and salinity on the ability of salmonella serotypes to form biofilm
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021792/
https://www.ncbi.nlm.nih.gov/pubmed/35464965
http://dx.doi.org/10.3389/fmicb.2022.821679
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