High concentrations of the anthelmintic diethylcarbamazine paralyze C. elegans independently of TRP-2

Diethylcarbamazine (DEC) has been used to treat lymphatic filariasis in tropical countries since the 1940s. Its mode of action is still unclear, with several reports suggesting a host immune system-mediated mechanism. We previously demonstrated that DEC causes transient spastic paralysis in the fila...

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Detalles Bibliográficos
Autores principales: Datta, Real, Robertson, Alan, Martin, Richard, Kashyap, Sudhanva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021882/
https://www.ncbi.nlm.nih.gov/pubmed/35622518
http://dx.doi.org/10.17912/micropub.biology.000548
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author Datta, Real
Robertson, Alan
Martin, Richard
Kashyap, Sudhanva
author_facet Datta, Real
Robertson, Alan
Martin, Richard
Kashyap, Sudhanva
author_sort Datta, Real
collection PubMed
description Diethylcarbamazine (DEC) has been used to treat lymphatic filariasis in tropical countries since the 1940s. Its mode of action is still unclear, with several reports suggesting a host immune system-mediated mechanism. We previously demonstrated that DEC causes transient spastic paralysis in the filarial nematode Brugia malayi due to the activation of TRP-2. Here we show that DEC causes transient paralysis in C. elegans at high concentrations and is 200x less potent compared to its effect on B. malayi. C. elegans trp-2(sy691) mutants are like the wild-type and only paralyzed by high concentrations of DEC. Our results demonstrate that high concentrations of DEC cause paralysis of C. elegans independent of TRP-2.
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spelling pubmed-90218822022-04-22 High concentrations of the anthelmintic diethylcarbamazine paralyze C. elegans independently of TRP-2 Datta, Real Robertson, Alan Martin, Richard Kashyap, Sudhanva MicroPubl Biol New Finding Diethylcarbamazine (DEC) has been used to treat lymphatic filariasis in tropical countries since the 1940s. Its mode of action is still unclear, with several reports suggesting a host immune system-mediated mechanism. We previously demonstrated that DEC causes transient spastic paralysis in the filarial nematode Brugia malayi due to the activation of TRP-2. Here we show that DEC causes transient paralysis in C. elegans at high concentrations and is 200x less potent compared to its effect on B. malayi. C. elegans trp-2(sy691) mutants are like the wild-type and only paralyzed by high concentrations of DEC. Our results demonstrate that high concentrations of DEC cause paralysis of C. elegans independent of TRP-2. Caltech Library 2022-04-20 /pmc/articles/PMC9021882/ /pubmed/35622518 http://dx.doi.org/10.17912/micropub.biology.000548 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Datta, Real
Robertson, Alan
Martin, Richard
Kashyap, Sudhanva
High concentrations of the anthelmintic diethylcarbamazine paralyze C. elegans independently of TRP-2
title High concentrations of the anthelmintic diethylcarbamazine paralyze C. elegans independently of TRP-2
title_full High concentrations of the anthelmintic diethylcarbamazine paralyze C. elegans independently of TRP-2
title_fullStr High concentrations of the anthelmintic diethylcarbamazine paralyze C. elegans independently of TRP-2
title_full_unstemmed High concentrations of the anthelmintic diethylcarbamazine paralyze C. elegans independently of TRP-2
title_short High concentrations of the anthelmintic diethylcarbamazine paralyze C. elegans independently of TRP-2
title_sort high concentrations of the anthelmintic diethylcarbamazine paralyze c. elegans independently of trp-2
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021882/
https://www.ncbi.nlm.nih.gov/pubmed/35622518
http://dx.doi.org/10.17912/micropub.biology.000548
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