Targeting DNA polymerase to DNA double-strand breaks reduces DNA deletion size and increases templated insertions generated by CRISPR/Cas9

Most insertions or deletions generated by CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) endonucleases are short (<25 bp), but unpredictable on-target long DNA deletions (>500 bp) can be observed. The possibility of generating long on-target...

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Detalles Bibliográficos
Autores principales: Yoo, Kyung W, Yadav, Manish Kumar, Song, Qianqian, Atala, Anthony, Lu, Baisong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Genome Integrity, Repair and Replication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023269/
https://www.ncbi.nlm.nih.gov/pubmed/35323942
http://dx.doi.org/10.1093/nar/gkac186

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023269/
https://www.ncbi.nlm.nih.gov/pubmed/35323942
http://dx.doi.org/10.1093/nar/gkac186

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