Cargando…

Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain

We examined the protective effects of esculetin and fucoidan against the neurotoxicity of ZnO NPs in rats. Ninety rats were divided into nine groups and pre-treated with esculetin or fucoidan 1 h before ZnO NP administration on a daily basis for 2 weeks. Serum and brain homogenates were examined by...

Descripción completa

Detalles Bibliográficos
Autores principales: Song, Woo-Ju, Kim, Jeongtae, Shin, Taekyun, Jeong, Myeong-Seon, Kim, Kil-Nam, Yun, Jang-Hyuk, Wie, Myung-Bok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025201/
https://www.ncbi.nlm.nih.gov/pubmed/35448455
http://dx.doi.org/10.3390/toxics10040194
_version_ 1784690810620477440
author Song, Woo-Ju
Kim, Jeongtae
Shin, Taekyun
Jeong, Myeong-Seon
Kim, Kil-Nam
Yun, Jang-Hyuk
Wie, Myung-Bok
author_facet Song, Woo-Ju
Kim, Jeongtae
Shin, Taekyun
Jeong, Myeong-Seon
Kim, Kil-Nam
Yun, Jang-Hyuk
Wie, Myung-Bok
author_sort Song, Woo-Ju
collection PubMed
description We examined the protective effects of esculetin and fucoidan against the neurotoxicity of ZnO NPs in rats. Ninety rats were divided into nine groups and pre-treated with esculetin or fucoidan 1 h before ZnO NP administration on a daily basis for 2 weeks. Serum and brain homogenates were examined by enzyme-linked immunosorbent assay (ELISA), and neurons, microglia, and astrocytes in the hippocampal region were examined with immunohistochemical analysis. The serum levels of interleukin-1-beta (IL-1β), 3-nitrotyrosine (3-NT), superoxide dismutase (SOD), and 8-hydroxy-2′-deoxyguanosine (8-OHdG) were altered in the ZnO NP treatment groups. Brain IL-1β and TNF-α levels were elevated after ZnO NP administration, and these effects were inhibited by esculetin and fucoidan. SOD, 8-OHdG, and acetylcholinesterase (AChE) levels in the brain were decreased after ZnO NP administration. The brain levels of beclin-1 and caspase-3 were elevated after ZnO NP treatment, and these effects were significantly ameliorated by esculetin and fucoidan. The number of reactive astrocytes measured by counting glial fibrillary acidic protein (GFAP)-positive cells, but not microglia, increased following ZnO NP treatment, and esculetin and fucoidan ameliorated the changes. Esculetin and fucoidan may be beneficial for preventing ZnO NP-mediated autophagy and apoptosis by the modulation of reactive astrocyte and proinflammatory cytokines in the rat brain.
format Online
Article
Text
id pubmed-9025201
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-90252012022-04-23 Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain Song, Woo-Ju Kim, Jeongtae Shin, Taekyun Jeong, Myeong-Seon Kim, Kil-Nam Yun, Jang-Hyuk Wie, Myung-Bok Toxics Article We examined the protective effects of esculetin and fucoidan against the neurotoxicity of ZnO NPs in rats. Ninety rats were divided into nine groups and pre-treated with esculetin or fucoidan 1 h before ZnO NP administration on a daily basis for 2 weeks. Serum and brain homogenates were examined by enzyme-linked immunosorbent assay (ELISA), and neurons, microglia, and astrocytes in the hippocampal region were examined with immunohistochemical analysis. The serum levels of interleukin-1-beta (IL-1β), 3-nitrotyrosine (3-NT), superoxide dismutase (SOD), and 8-hydroxy-2′-deoxyguanosine (8-OHdG) were altered in the ZnO NP treatment groups. Brain IL-1β and TNF-α levels were elevated after ZnO NP administration, and these effects were inhibited by esculetin and fucoidan. SOD, 8-OHdG, and acetylcholinesterase (AChE) levels in the brain were decreased after ZnO NP administration. The brain levels of beclin-1 and caspase-3 were elevated after ZnO NP treatment, and these effects were significantly ameliorated by esculetin and fucoidan. The number of reactive astrocytes measured by counting glial fibrillary acidic protein (GFAP)-positive cells, but not microglia, increased following ZnO NP treatment, and esculetin and fucoidan ameliorated the changes. Esculetin and fucoidan may be beneficial for preventing ZnO NP-mediated autophagy and apoptosis by the modulation of reactive astrocyte and proinflammatory cytokines in the rat brain. MDPI 2022-04-16 /pmc/articles/PMC9025201/ /pubmed/35448455 http://dx.doi.org/10.3390/toxics10040194 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Woo-Ju
Kim, Jeongtae
Shin, Taekyun
Jeong, Myeong-Seon
Kim, Kil-Nam
Yun, Jang-Hyuk
Wie, Myung-Bok
Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain
title Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain
title_full Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain
title_fullStr Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain
title_full_unstemmed Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain
title_short Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain
title_sort esculetin and fucoidan attenuate autophagy and apoptosis induced by zinc oxide nanoparticles through modulating reactive astrocyte and proinflammatory cytokines in the rat brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025201/
https://www.ncbi.nlm.nih.gov/pubmed/35448455
http://dx.doi.org/10.3390/toxics10040194
work_keys_str_mv AT songwooju esculetinandfucoidanattenuateautophagyandapoptosisinducedbyzincoxidenanoparticlesthroughmodulatingreactiveastrocyteandproinflammatorycytokinesintheratbrain
AT kimjeongtae esculetinandfucoidanattenuateautophagyandapoptosisinducedbyzincoxidenanoparticlesthroughmodulatingreactiveastrocyteandproinflammatorycytokinesintheratbrain
AT shintaekyun esculetinandfucoidanattenuateautophagyandapoptosisinducedbyzincoxidenanoparticlesthroughmodulatingreactiveastrocyteandproinflammatorycytokinesintheratbrain
AT jeongmyeongseon esculetinandfucoidanattenuateautophagyandapoptosisinducedbyzincoxidenanoparticlesthroughmodulatingreactiveastrocyteandproinflammatorycytokinesintheratbrain
AT kimkilnam esculetinandfucoidanattenuateautophagyandapoptosisinducedbyzincoxidenanoparticlesthroughmodulatingreactiveastrocyteandproinflammatorycytokinesintheratbrain
AT yunjanghyuk esculetinandfucoidanattenuateautophagyandapoptosisinducedbyzincoxidenanoparticlesthroughmodulatingreactiveastrocyteandproinflammatorycytokinesintheratbrain
AT wiemyungbok esculetinandfucoidanattenuateautophagyandapoptosisinducedbyzincoxidenanoparticlesthroughmodulatingreactiveastrocyteandproinflammatorycytokinesintheratbrain