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Developing CRISPR/Cas9-Mediated Fluorescent Reporter Human Pluripotent Stem-Cell Lines for High-Content Screening
Application of the CRISPR/Cas9 system to knock in fluorescent proteins to endogenous genes of interest in human pluripotent stem cells (hPSCs) has the potential to facilitate hPSC-based disease modeling, drug screening, and optimization of transplantation therapy. To evaluate the capability of fluor...
Autores principales: | Vojnits, Kinga, Nakanishi, Mio, Porras, Deanna, Kim, Yeonjoon, Feng, Zhuohang, Golubeva, Diana, Bhatia, Mick |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025795/ https://www.ncbi.nlm.nih.gov/pubmed/35458632 http://dx.doi.org/10.3390/molecules27082434 |
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