High-yield overproduction and purification of human aquaporins from Pichia pastoris

Aquaporins (AQPs) are membrane-bound water channels that play crucial roles in maintaining the water homeostasis of the human body. Here, we present a protocol for high-yield recombinant expression of human AQPs in the methylotropic yeast Pichia pastoris and subsequent AQP purification. The protocol...

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Detalles Bibliográficos
Autores principales: Al-Jubair, Tamim, Steffen, Jonas Hyld, Missel, Julie Winkel, Kitchen, Philip, Salman, Mootaz M., Bill, Roslyn M., Gourdon, Pontus, Törnroth-Horsefield, Susanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026565/
https://www.ncbi.nlm.nih.gov/pubmed/35463467
http://dx.doi.org/10.1016/j.xpro.2022.101298

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026565/
https://www.ncbi.nlm.nih.gov/pubmed/35463467
http://dx.doi.org/10.1016/j.xpro.2022.101298

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