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Add and Go: FRET Acceptor for Live-Cell Measurements Modulated by Externally Provided Ligand
A substantial number of genetically encoded fluorescent sensors rely on the changes in FRET efficiency between fluorescent cores, measured in ratiometric mode, with acceptor photobleaching or by changes in fluorescence lifetime. We report on a modulated FRET acceptor allowing for simplified one-chan...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026985/ https://www.ncbi.nlm.nih.gov/pubmed/35457212 http://dx.doi.org/10.3390/ijms23084396 |