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Quality Control of Bacterial Extracellular Vesicles with Total Protein Content Assay, Nanoparticles Tracking Analysis, and Capillary Electrophoresis

Extracellular vesicles (EVs) were isolated from Pectobacterium zantedeschiae culturing media using direct ultracentrifugation (UC), iodixanol cushion ultracentrifugation (ICUC), and iodixanol density gradient ultracentrifugation (IDGUC) techniques. The isolates were characterized with total protein...

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Detalles Bibliográficos
Autores principales: Steć, Aleksandra, Jońca, Joanna, Waleron, Krzysztof, Waleron, Małgorzata, Płoska, Agata, Kalinowski, Leszek, Wielgomas, Bartosz, Dziomba, Szymon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028362/
https://www.ncbi.nlm.nih.gov/pubmed/35457164
http://dx.doi.org/10.3390/ijms23084347