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Pharmacokinetic Imaging Using (99m)Tc-Mebrofenin to Untangle the Pattern of Hepatocyte Transporter Disruptions Induced by Endotoxemia in Rats
Endotoxemia-induced inflammation may impact the activity of hepatocyte transporters, which control the hepatobiliary elimination of drugs and bile acids. (99m)Tc-mebrofenin is a non-metabolized substrate of transporters expressed at the different poles of hepatocytes. (99m)Tc-mebrofenin imaging was...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028474/ https://www.ncbi.nlm.nih.gov/pubmed/35455390 http://dx.doi.org/10.3390/ph15040392 |
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author | Marie, Solène Hernández-Lozano, Irene Le Vée, Marc Breuil, Louise Saba, Wadad Goislard, Maud Goutal, Sébastien Truillet, Charles Langer, Oliver Fardel, Olivier Tournier, Nicolas |
author_facet | Marie, Solène Hernández-Lozano, Irene Le Vée, Marc Breuil, Louise Saba, Wadad Goislard, Maud Goutal, Sébastien Truillet, Charles Langer, Oliver Fardel, Olivier Tournier, Nicolas |
author_sort | Marie, Solène |
collection | PubMed |
description | Endotoxemia-induced inflammation may impact the activity of hepatocyte transporters, which control the hepatobiliary elimination of drugs and bile acids. (99m)Tc-mebrofenin is a non-metabolized substrate of transporters expressed at the different poles of hepatocytes. (99m)Tc-mebrofenin imaging was performed in rats after the injection of lipopolysaccharide (LPS). Changes in transporter expression were assessed using quantitative polymerase chain reaction of resected liver samples. Moreover, the particular impact of pharmacokinetic drug–drug interactions in the context of endotoxemia was investigated using rifampicin (40 mg/kg), a potent inhibitor of hepatocyte transporters. LPS increased (99m)Tc-mebrofenin exposure in the liver (1.7 ± 0.4-fold). Kinetic modeling revealed that endotoxemia did not impact the blood-to-liver uptake of (99m)Tc-mebrofenin, which is mediated by organic anion-transporting polypeptide (Oatp) transporters. However, liver-to-bile and liver-to-blood efflux rates were dramatically decreased, leading to liver accumulation. The transcriptomic profile of hepatocyte transporters consistently showed a downregulation of multidrug resistance-associated proteins 2 and 3 (Mrp2 and Mrp3), which mediate the canalicular and sinusoidal efflux of (99m)Tc-mebrofenin in hepatocytes, respectively. Rifampicin effectively blocked both the Oatp-mediated influx and the Mrp2/3-related efflux of (99m)Tc-mebrofenin. The additive impact of endotoxemia and rifampicin led to a 3.0 ± 1.3-fold increase in blood exposure compared with healthy non-treated animals. (99m)Tc-mebrofenin imaging is useful to investigate disease-associated change in hepatocyte transporter function. |
format | Online Article Text |
id | pubmed-9028474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90284742022-04-23 Pharmacokinetic Imaging Using (99m)Tc-Mebrofenin to Untangle the Pattern of Hepatocyte Transporter Disruptions Induced by Endotoxemia in Rats Marie, Solène Hernández-Lozano, Irene Le Vée, Marc Breuil, Louise Saba, Wadad Goislard, Maud Goutal, Sébastien Truillet, Charles Langer, Oliver Fardel, Olivier Tournier, Nicolas Pharmaceuticals (Basel) Article Endotoxemia-induced inflammation may impact the activity of hepatocyte transporters, which control the hepatobiliary elimination of drugs and bile acids. (99m)Tc-mebrofenin is a non-metabolized substrate of transporters expressed at the different poles of hepatocytes. (99m)Tc-mebrofenin imaging was performed in rats after the injection of lipopolysaccharide (LPS). Changes in transporter expression were assessed using quantitative polymerase chain reaction of resected liver samples. Moreover, the particular impact of pharmacokinetic drug–drug interactions in the context of endotoxemia was investigated using rifampicin (40 mg/kg), a potent inhibitor of hepatocyte transporters. LPS increased (99m)Tc-mebrofenin exposure in the liver (1.7 ± 0.4-fold). Kinetic modeling revealed that endotoxemia did not impact the blood-to-liver uptake of (99m)Tc-mebrofenin, which is mediated by organic anion-transporting polypeptide (Oatp) transporters. However, liver-to-bile and liver-to-blood efflux rates were dramatically decreased, leading to liver accumulation. The transcriptomic profile of hepatocyte transporters consistently showed a downregulation of multidrug resistance-associated proteins 2 and 3 (Mrp2 and Mrp3), which mediate the canalicular and sinusoidal efflux of (99m)Tc-mebrofenin in hepatocytes, respectively. Rifampicin effectively blocked both the Oatp-mediated influx and the Mrp2/3-related efflux of (99m)Tc-mebrofenin. The additive impact of endotoxemia and rifampicin led to a 3.0 ± 1.3-fold increase in blood exposure compared with healthy non-treated animals. (99m)Tc-mebrofenin imaging is useful to investigate disease-associated change in hepatocyte transporter function. MDPI 2022-03-24 /pmc/articles/PMC9028474/ /pubmed/35455390 http://dx.doi.org/10.3390/ph15040392 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Marie, Solène Hernández-Lozano, Irene Le Vée, Marc Breuil, Louise Saba, Wadad Goislard, Maud Goutal, Sébastien Truillet, Charles Langer, Oliver Fardel, Olivier Tournier, Nicolas Pharmacokinetic Imaging Using (99m)Tc-Mebrofenin to Untangle the Pattern of Hepatocyte Transporter Disruptions Induced by Endotoxemia in Rats |
title | Pharmacokinetic Imaging Using (99m)Tc-Mebrofenin to Untangle the Pattern of Hepatocyte Transporter Disruptions Induced by Endotoxemia in Rats |
title_full | Pharmacokinetic Imaging Using (99m)Tc-Mebrofenin to Untangle the Pattern of Hepatocyte Transporter Disruptions Induced by Endotoxemia in Rats |
title_fullStr | Pharmacokinetic Imaging Using (99m)Tc-Mebrofenin to Untangle the Pattern of Hepatocyte Transporter Disruptions Induced by Endotoxemia in Rats |
title_full_unstemmed | Pharmacokinetic Imaging Using (99m)Tc-Mebrofenin to Untangle the Pattern of Hepatocyte Transporter Disruptions Induced by Endotoxemia in Rats |
title_short | Pharmacokinetic Imaging Using (99m)Tc-Mebrofenin to Untangle the Pattern of Hepatocyte Transporter Disruptions Induced by Endotoxemia in Rats |
title_sort | pharmacokinetic imaging using (99m)tc-mebrofenin to untangle the pattern of hepatocyte transporter disruptions induced by endotoxemia in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028474/ https://www.ncbi.nlm.nih.gov/pubmed/35455390 http://dx.doi.org/10.3390/ph15040392 |
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