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Continuous Percoll Gradient Centrifugation of Erythrocytes—Explanation of Cellular Bands and Compromised Age Separation

(1) Background: When red blood cells are centrifuged in a continuous Percoll-based density gradient, they form discrete bands. While this is a popular approach for red blood cell age separation, the mechanisms involved in banding were unknown. (2) Methods: Percoll centrifugations of red blood cells...

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Detalles Bibliográficos
Autores principales: Maurer, Felix, John, Thomas, Makhro, Asya, Bogdanova, Anna, Minetti, Giampaolo, Wagner, Christian, Kaestner, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028966/
https://www.ncbi.nlm.nih.gov/pubmed/35455975
http://dx.doi.org/10.3390/cells11081296