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Use of Cocktail of Bacteriophage for Salmonella Typhimurium Control in Chicken Meat

Foodborne diseases are extremely relevant and constitute an area of alert for public health authorities due to the high impact and number of people affected each year. The food industry has implemented microbiological control plans that ensure the quality and safety of its products; however, due to...

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Autores principales: Aguilera, Matías, Martínez, Sofía, Tello, Mario, Gallardo, María José, García, Verónica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029022/
https://www.ncbi.nlm.nih.gov/pubmed/35454751
http://dx.doi.org/10.3390/foods11081164
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author Aguilera, Matías
Martínez, Sofía
Tello, Mario
Gallardo, María José
García, Verónica
author_facet Aguilera, Matías
Martínez, Sofía
Tello, Mario
Gallardo, María José
García, Verónica
author_sort Aguilera, Matías
collection PubMed
description Foodborne diseases are extremely relevant and constitute an area of alert for public health authorities due to the high impact and number of people affected each year. The food industry has implemented microbiological control plans that ensure the quality and safety of its products; however, due to the high prevalence of foodborne diseases, the industry requires new microbiological control systems. One of the main causative agents of diseases transmitted by poultry meat is the bacterium Salmonella enterica. Disinfectants, antibiotics, and vaccines are used to control this pathogen. However, they have not been efficient in the total elimination of these bacteria, with numerous outbreaks caused by this bacterium observed today, in addition to the increase in antibiotic-resistant bacteria. The search for new technologies to reduce microbial contamination in the poultry industry continues to be a necessity and the use of lytic bacteriophages is one of the new solutions. In this study, 20 bacteriophages were isolated for Salmonella spp. obtained from natural environments and cocktails composed of five of them were designed, where three belonged to the Siphoviridae family and two to the Microviridae family. This cocktail was tested on chicken meat infected with Salmonella Typhimurium at 10 °C, where it was found that this cocktail was capable of decreasing 1.4 logarithmic units at 48 h compared to the control.
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spelling pubmed-90290222022-04-23 Use of Cocktail of Bacteriophage for Salmonella Typhimurium Control in Chicken Meat Aguilera, Matías Martínez, Sofía Tello, Mario Gallardo, María José García, Verónica Foods Article Foodborne diseases are extremely relevant and constitute an area of alert for public health authorities due to the high impact and number of people affected each year. The food industry has implemented microbiological control plans that ensure the quality and safety of its products; however, due to the high prevalence of foodborne diseases, the industry requires new microbiological control systems. One of the main causative agents of diseases transmitted by poultry meat is the bacterium Salmonella enterica. Disinfectants, antibiotics, and vaccines are used to control this pathogen. However, they have not been efficient in the total elimination of these bacteria, with numerous outbreaks caused by this bacterium observed today, in addition to the increase in antibiotic-resistant bacteria. The search for new technologies to reduce microbial contamination in the poultry industry continues to be a necessity and the use of lytic bacteriophages is one of the new solutions. In this study, 20 bacteriophages were isolated for Salmonella spp. obtained from natural environments and cocktails composed of five of them were designed, where three belonged to the Siphoviridae family and two to the Microviridae family. This cocktail was tested on chicken meat infected with Salmonella Typhimurium at 10 °C, where it was found that this cocktail was capable of decreasing 1.4 logarithmic units at 48 h compared to the control. MDPI 2022-04-17 /pmc/articles/PMC9029022/ /pubmed/35454751 http://dx.doi.org/10.3390/foods11081164 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aguilera, Matías
Martínez, Sofía
Tello, Mario
Gallardo, María José
García, Verónica
Use of Cocktail of Bacteriophage for Salmonella Typhimurium Control in Chicken Meat
title Use of Cocktail of Bacteriophage for Salmonella Typhimurium Control in Chicken Meat
title_full Use of Cocktail of Bacteriophage for Salmonella Typhimurium Control in Chicken Meat
title_fullStr Use of Cocktail of Bacteriophage for Salmonella Typhimurium Control in Chicken Meat
title_full_unstemmed Use of Cocktail of Bacteriophage for Salmonella Typhimurium Control in Chicken Meat
title_short Use of Cocktail of Bacteriophage for Salmonella Typhimurium Control in Chicken Meat
title_sort use of cocktail of bacteriophage for salmonella typhimurium control in chicken meat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029022/
https://www.ncbi.nlm.nih.gov/pubmed/35454751
http://dx.doi.org/10.3390/foods11081164
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