Cargando…

Lactobacillus salivarius CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens

Lactobacillus strains with fine probiotic properties are continuously needed in the laying hen industry to improve the animals’ gut health and production performance. In this study, we isolated 57 Lactobacillus strains from the gut microbiota of 17 different chicken breeds in China. We characterized...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Chang, Wei, Fuxiao, Yang, Xinyue, Feng, Yuqing, Liu, Dan, Hu, Yongfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029475/
https://www.ncbi.nlm.nih.gov/pubmed/35456777
http://dx.doi.org/10.3390/microorganisms10040726
_version_ 1784691887299362816
author Xu, Chang
Wei, Fuxiao
Yang, Xinyue
Feng, Yuqing
Liu, Dan
Hu, Yongfei
author_facet Xu, Chang
Wei, Fuxiao
Yang, Xinyue
Feng, Yuqing
Liu, Dan
Hu, Yongfei
author_sort Xu, Chang
collection PubMed
description Lactobacillus strains with fine probiotic properties are continuously needed in the laying hen industry to improve the animals’ gut health and production performance. In this study, we isolated 57 Lactobacillus strains from the gut microbiota of 17 different chicken breeds in China. We characterized the probiotic features of these isolates, and evaluated the effects of a selected strain, Lactobacillus salivarius CML352, on the production performance and gut health of the late-phase laying hens. The results showed that the isolates varied much in probiotic properties, among which L. salivarius CML352 displayed high acid and bile salt tolerance, high hydrophobicity, auto-aggregation, and antibacterial activities. Whole genome sequencing analysis showed that CML352 was closely related to a strain isolated from human fecal samples, but had different functional potentials. Dietary supplementary of L. salivarius CML352 significantly reduced the Firmicutes to Bacteroidetes ratio, increased the expression of Muc-2, and decreased the expression of MyD88, IFN-γ, and TLR-4. Furthermore, strain CML352 reduced the birds’ abdominal fat deposition, and improved egg quality. Taken together, this study indicated that the newly isolated L. salivarius strain might be a worthy probiotic with positive impacts on the intestinal health and production performance of late-phase laying hens.
format Online
Article
Text
id pubmed-9029475
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-90294752022-04-23 Lactobacillus salivarius CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens Xu, Chang Wei, Fuxiao Yang, Xinyue Feng, Yuqing Liu, Dan Hu, Yongfei Microorganisms Article Lactobacillus strains with fine probiotic properties are continuously needed in the laying hen industry to improve the animals’ gut health and production performance. In this study, we isolated 57 Lactobacillus strains from the gut microbiota of 17 different chicken breeds in China. We characterized the probiotic features of these isolates, and evaluated the effects of a selected strain, Lactobacillus salivarius CML352, on the production performance and gut health of the late-phase laying hens. The results showed that the isolates varied much in probiotic properties, among which L. salivarius CML352 displayed high acid and bile salt tolerance, high hydrophobicity, auto-aggregation, and antibacterial activities. Whole genome sequencing analysis showed that CML352 was closely related to a strain isolated from human fecal samples, but had different functional potentials. Dietary supplementary of L. salivarius CML352 significantly reduced the Firmicutes to Bacteroidetes ratio, increased the expression of Muc-2, and decreased the expression of MyD88, IFN-γ, and TLR-4. Furthermore, strain CML352 reduced the birds’ abdominal fat deposition, and improved egg quality. Taken together, this study indicated that the newly isolated L. salivarius strain might be a worthy probiotic with positive impacts on the intestinal health and production performance of late-phase laying hens. MDPI 2022-03-28 /pmc/articles/PMC9029475/ /pubmed/35456777 http://dx.doi.org/10.3390/microorganisms10040726 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Chang
Wei, Fuxiao
Yang, Xinyue
Feng, Yuqing
Liu, Dan
Hu, Yongfei
Lactobacillus salivarius CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens
title Lactobacillus salivarius CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens
title_full Lactobacillus salivarius CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens
title_fullStr Lactobacillus salivarius CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens
title_full_unstemmed Lactobacillus salivarius CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens
title_short Lactobacillus salivarius CML352 Isolated from Chinese Local Breed Chicken Modulates the Gut Microbiota and Improves Intestinal Health and Egg Quality in Late-Phase Laying Hens
title_sort lactobacillus salivarius cml352 isolated from chinese local breed chicken modulates the gut microbiota and improves intestinal health and egg quality in late-phase laying hens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029475/
https://www.ncbi.nlm.nih.gov/pubmed/35456777
http://dx.doi.org/10.3390/microorganisms10040726
work_keys_str_mv AT xuchang lactobacillussalivariuscml352isolatedfromchineselocalbreedchickenmodulatesthegutmicrobiotaandimprovesintestinalhealthandeggqualityinlatephaselayinghens
AT weifuxiao lactobacillussalivariuscml352isolatedfromchineselocalbreedchickenmodulatesthegutmicrobiotaandimprovesintestinalhealthandeggqualityinlatephaselayinghens
AT yangxinyue lactobacillussalivariuscml352isolatedfromchineselocalbreedchickenmodulatesthegutmicrobiotaandimprovesintestinalhealthandeggqualityinlatephaselayinghens
AT fengyuqing lactobacillussalivariuscml352isolatedfromchineselocalbreedchickenmodulatesthegutmicrobiotaandimprovesintestinalhealthandeggqualityinlatephaselayinghens
AT liudan lactobacillussalivariuscml352isolatedfromchineselocalbreedchickenmodulatesthegutmicrobiotaandimprovesintestinalhealthandeggqualityinlatephaselayinghens
AT huyongfei lactobacillussalivariuscml352isolatedfromchineselocalbreedchickenmodulatesthegutmicrobiotaandimprovesintestinalhealthandeggqualityinlatephaselayinghens