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Targeted esterase-induced dye (TED) loading supports direct calcium imaging in eukaryotic cell-free systems

Calcium imaging is an important functional tool for analysing ion channels, transporters and pumps for drug screening in living cells. Depicted eukaryotic cell-free systems utilize microsomes, derived from the endoplasmic reticulum to incorporate the synthesized membrane proteins-like ion channels....

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Detalles Bibliográficos
Autores principales: Dhandapani, Priyavathi, Dondapati, Srujan Kumar, Zemella, Anne, Bräuer, Dennis, Wüstenhagen, Doreen Anja, Mergler, Stefan, Kubick, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030739/
https://www.ncbi.nlm.nih.gov/pubmed/35479141
http://dx.doi.org/10.1039/d0ra08397f