Cargando…
Targeted esterase-induced dye (TED) loading supports direct calcium imaging in eukaryotic cell-free systems
Calcium imaging is an important functional tool for analysing ion channels, transporters and pumps for drug screening in living cells. Depicted eukaryotic cell-free systems utilize microsomes, derived from the endoplasmic reticulum to incorporate the synthesized membrane proteins-like ion channels....
Autores principales: | Dhandapani, Priyavathi, Dondapati, Srujan Kumar, Zemella, Anne, Bräuer, Dennis, Wüstenhagen, Doreen Anja, Mergler, Stefan, Kubick, Stefan |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030739/ https://www.ncbi.nlm.nih.gov/pubmed/35479141 http://dx.doi.org/10.1039/d0ra08397f |
Ejemplares similares
-
Functional Reconstitution of Membrane Proteins Derived From Eukaryotic Cell-Free Systems
por: Dondapati, Srujan Kumar, et al.
Publicado: (2019) -
Accelerating the Production of Druggable Targets: Eukaryotic Cell-Free Systems Come into Focus
por: Thoring, Lena, et al.
Publicado: (2019) -
Mammalian cell-free protein expression promotes the functional characterization of the tripartite non-hemolytic enterotoxin from Bacillus cereus
por: Ramm, Franziska, et al.
Publicado: (2020) -
Evaluation of the Ion Channel Assembly in a Eukaryotic Cell-Free System Focusing on Two-Pore Domain Potassium Channels K(2P)
por: Ullrich, Jessica, et al.
Publicado: (2023) -
Cell‐free production of pore forming toxins: Functional analysis of thermostable direct hemolysin from Vibrio parahaemolyticus
por: Dondapati, Srujan Kumar, et al.
Publicado: (2017)