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A Non-Hazardous Deparaffinization Protocol Enables Quantitative Proteomics of Core Needle Biopsy-Sized Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens
Most human tumor tissues that are obtained for pathology and diagnostic purposes are formalin-fixed and paraffin-embedded (FFPE). To perform quantitative proteomics of FFPE samples, paraffin has to be removed and formalin-induced crosslinks have to be reversed prior to proteolytic digestion. A centr...
Autores principales: | Mitsa, Georgia, Guo, Qianyu, Goncalves, Christophe, Preston, Samuel E. J., Lacasse, Vincent, Aguilar-Mahecha, Adriana, Benlimame, Naciba, Basik, Mark, Spatz, Alan, Batist, Gerald, Miller, Wilson H., del Rincon, Sonia V., Zahedi, René P., Borchers, Christoph H. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031572/ https://www.ncbi.nlm.nih.gov/pubmed/35457260 http://dx.doi.org/10.3390/ijms23084443 |
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