Microfluidic One-Pot Digital Droplet FISH Using LNA/DNA Molecular Beacons for Bacteria Detection and Absolute Quantification

We demonstrate detection and quantification of bacterial load with a novel microfluidic one-pot wash-free fluorescence in situ hybridization (FISH) assay in droplets. The method offers minimal manual workload by only requiring mixing of the sample with reagents and loading it into a microfluidic car...

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Detalles Bibliográficos
Autores principales: Kao, Yu-Ting, Calabrese, Silvia, Borst, Nadine, Lehnert, Michael, Lai, Yu-Kai, Schlenker, Franziska, Juelg, Peter, Zengerle, Roland, Garstecki, Piotr, von Stetten, Felix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032532/
https://www.ncbi.nlm.nih.gov/pubmed/35448297
http://dx.doi.org/10.3390/bios12040237

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032532/
https://www.ncbi.nlm.nih.gov/pubmed/35448297
http://dx.doi.org/10.3390/bios12040237

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