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EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling

Gliomas are the most common intrinsic brain tumors in adults, and in accordance with their clinical behavior and patients’ outcome, they are graded by the World Health Organization (WHO) classification of brain tumors. One very interesting candidate for targeted tumor therapy may be epidermal growth...

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Autores principales: Hölzl, Dorothee, Hutarew, Georg, Zellinger, Barbara, Alinger-Scharinger, Beate, Schlicker, Hans U., Schwartz, Christoph, Sotlar, Karl, Kraus, Theo F. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033057/
https://www.ncbi.nlm.nih.gov/pubmed/35453544
http://dx.doi.org/10.3390/biomedicines10040794
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author Hölzl, Dorothee
Hutarew, Georg
Zellinger, Barbara
Alinger-Scharinger, Beate
Schlicker, Hans U.
Schwartz, Christoph
Sotlar, Karl
Kraus, Theo F. J.
author_facet Hölzl, Dorothee
Hutarew, Georg
Zellinger, Barbara
Alinger-Scharinger, Beate
Schlicker, Hans U.
Schwartz, Christoph
Sotlar, Karl
Kraus, Theo F. J.
author_sort Hölzl, Dorothee
collection PubMed
description Gliomas are the most common intrinsic brain tumors in adults, and in accordance with their clinical behavior and patients’ outcome, they are graded by the World Health Organization (WHO) classification of brain tumors. One very interesting candidate for targeted tumor therapy may be epidermal growth factor receptor (EGFR) amplification. Here, we performed an integrated comparative analysis of EGFR amplification in 34 glioma samples using standard fluorescence in situ hybridization (FISH) and Illumina EPIC Infinium Methylation Bead Chip and correlated results with molecular glioma hallmarks. We found that the EPIC analysis showed the same power of detecting EGFR amplification compared with FISH. EGFR amplification was detectable in high-grade gliomas (25%). Moreover, EGFR amplification was found to be present solely in IDH wildtype gliomas (26%) and TERT mutated gliomas (27%), occurring independently of MGMT promoter methylation status and being mutually exclusive with 1p/19q codeletion (LOH). In summary, EPIC Bead Chip analysis is a reliable tool for detecting EGFR amplification and is comparable with the standard method FISH. EGFR amplification is a phenomenon of IDH wildtype TERT mutated high-grade gliomas.
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spelling pubmed-90330572022-04-23 EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling Hölzl, Dorothee Hutarew, Georg Zellinger, Barbara Alinger-Scharinger, Beate Schlicker, Hans U. Schwartz, Christoph Sotlar, Karl Kraus, Theo F. J. Biomedicines Article Gliomas are the most common intrinsic brain tumors in adults, and in accordance with their clinical behavior and patients’ outcome, they are graded by the World Health Organization (WHO) classification of brain tumors. One very interesting candidate for targeted tumor therapy may be epidermal growth factor receptor (EGFR) amplification. Here, we performed an integrated comparative analysis of EGFR amplification in 34 glioma samples using standard fluorescence in situ hybridization (FISH) and Illumina EPIC Infinium Methylation Bead Chip and correlated results with molecular glioma hallmarks. We found that the EPIC analysis showed the same power of detecting EGFR amplification compared with FISH. EGFR amplification was detectable in high-grade gliomas (25%). Moreover, EGFR amplification was found to be present solely in IDH wildtype gliomas (26%) and TERT mutated gliomas (27%), occurring independently of MGMT promoter methylation status and being mutually exclusive with 1p/19q codeletion (LOH). In summary, EPIC Bead Chip analysis is a reliable tool for detecting EGFR amplification and is comparable with the standard method FISH. EGFR amplification is a phenomenon of IDH wildtype TERT mutated high-grade gliomas. MDPI 2022-03-29 /pmc/articles/PMC9033057/ /pubmed/35453544 http://dx.doi.org/10.3390/biomedicines10040794 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hölzl, Dorothee
Hutarew, Georg
Zellinger, Barbara
Alinger-Scharinger, Beate
Schlicker, Hans U.
Schwartz, Christoph
Sotlar, Karl
Kraus, Theo F. J.
EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling
title EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling
title_full EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling
title_fullStr EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling
title_full_unstemmed EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling
title_short EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling
title_sort egfr amplification is a phenomenon of idh wildtype and tert mutated high-grade glioma: an integrated analysis using fluorescence in situ hybridization and dna methylome profiling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033057/
https://www.ncbi.nlm.nih.gov/pubmed/35453544
http://dx.doi.org/10.3390/biomedicines10040794
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