Efficient generation of locus-specific human CAR-T cells with CRISPR/cCas12a

We recently developed a system to create human chimeric antigen receptor (CAR)-T cells using conjugated Cas12a (cCas12a) in which Cas12a is covalently linked to its CRISPR RNA (crRNA). This protocol describes site-specific modification of Cas12a and the preparation of Cas12a-crRNA complex using bio-...

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Detalles Bibliográficos
Autores principales: Ling, Xinyu, Chang, Liying, Chen, Heqi, Liu, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038777/
https://www.ncbi.nlm.nih.gov/pubmed/35496795
http://dx.doi.org/10.1016/j.xpro.2022.101321

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038777/
https://www.ncbi.nlm.nih.gov/pubmed/35496795
http://dx.doi.org/10.1016/j.xpro.2022.101321

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