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Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast

Chemically induced dimerization (CID) is a useful tool for artificially inducing protein-protein interactions. Although CID has been used extensively for live-cell microscopy applications in mammalian systems, it is rarely utilized in yeast cell biology studies. Here, we present a step-by-step proto...

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Detalles Bibliográficos
Autores principales: Lamb, Andrew K., Di Pietro, Santiago M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038778/
https://www.ncbi.nlm.nih.gov/pubmed/35496798
http://dx.doi.org/10.1016/j.xpro.2022.101323